Emergency and Critical Care Center, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
Int J Mol Med. 2019 Dec;44(6):2003-2014. doi: 10.3892/ijmm.2019.4380. Epub 2019 Oct 22.
In the present study, we examined the function of microRNA (miR)‑146a‑5p in patients with refractory Mycoplasma pneumoniae pneumonia. In brief, the expression of miR‑146a‑5p was reduced in patients with refractory Mycoplasma pneumoniae pneumonia. Downregulation of miR‑146a‑5p reduced inflammation in an in vitro model of refractory Mycoplasma pneumoniae pneumonia, whilst overexpression of miR‑146a‑5p promoted inflammation. Downregulation of miR‑146a‑5p induced the protein expression of ATP‑binding cassette subfamily G member 1 (ABCG1) and interleukin 1 receptor‑associated kinase 1 (IRAK‑1), while suppressed expression was observed of the aforementioned proteins following overexpression of miR‑146a‑5p in an in vitro model of refractory Mycoplasma pneumoniae pneumonia. The administration of small interfering RNA against RXR or IRAK‑1 attenuated the effects of miR‑146a‑5p on inflammation in an in vitro model of refractory Mycoplasma pneumoniae pneumonia. Collectively, these results suggested that miR‑146a‑5p reduced ABCG1 expression in refractory Mycoplasma pneumoniae pneumonia via downregulation of IRAK‑1.
在本研究中，我们研究了微小 RNA（miR）-146a-5p 在难治性肺炎支原体肺炎患者中的功能。简而言之，难治性肺炎支原体肺炎患者的 miR-146a-5p 表达降低。miR-146a-5p 的下调减少了难治性肺炎支原体肺炎体外模型中的炎症，而 miR-146a-5p 的过表达则促进了炎症。miR-146a-5p 的下调诱导了 ABCG1 和白细胞介素 1 受体相关激酶 1（IRAK-1）的蛋白表达，而在难治性肺炎支原体肺炎体外模型中过表达 miR-146a-5p 时则观察到上述蛋白的表达受到抑制。针对 RXR 或 IRAK-1 的小干扰 RNA 的给药减轻了 miR-146a-5p 在难治性肺炎支原体肺炎体外模型中对炎症的影响。综上所述，这些结果表明，miR-146a-5p 通过下调 IRAK-1 减少了难治性肺炎支原体肺炎中的 ABCG1 表达。
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