儿童肺炎支原体肺炎外周血 microR-146a 和 microR-29c 的表达及其临床价值。
Peripheral blood microR-146a and microR-29c expression in children with Mycoplasma pneumoniae pneumonia and its clinical value.
机构信息
Department of Pediatric Medicine, Affiliated Hospital of Chengde Medical College, Chengde, 067000, China.
出版信息
Ital J Pediatr. 2023 Sep 13;49(1):119. doi: 10.1186/s13052-023-01500-0.
BACKGROUND
We investigated changes in microR-29c and microR-146a expression in the serum of children with Mycoplasma pneumoniae pneumonia, analysed their relationship with inflammatory factors and disease severity, and evaluated their diagnostic significance.
METHODS
Fifty-six children with Mycoplasma pneumoniae pneumonia were enrolled as the Mycoplasma pneumoniae pneumonia group; 37 healthy children were enrolled as the control group. The microR-29c or microR-146a serum expression levels were determined using real-time quantitative reverse transcription polymerase chain reaction. Interleukin-17, tumour necrosis factor-alpha, and interleukin-1 beta levels were detected using enzyme-linked immunosorbent assay. The correlation between serum microR-29c or microR-146a expression and inflammatory factors was analysed using the Pearson's method. Receiver operating characteristic curves were used to evaluate the diagnostic value of serum microR-29c, microR-146a, and their combined detection in Mycoplasma pneumoniae pneumonia.
RESULTS
Compared with that in healthy children, the microR-29c and microR-146a serum levels were significantly downregulated in children with Mycoplasma pneumoniae pneumonia; the decrease was more obvious in children with severe cases than that in those with mild cases. In addition, microR-29c and microR-146a were negatively correlated with increased expression of interleukin-17, tumour necrosis factor-alpha, and interleukin-1 beta. Receiver operating characteristic curves showed that a combination of microR-29c and microR-146a was highly suitable for diagnosing Mycoplasma pneumoniae pneumonia.
CONCLUSION
Serum microR-29c and microR-146a were underexpressed in children with Mycoplasma pneumoniae pneumonia, and diagnostic accuracy was significantly improved with combined microR-29c and microR-146a detection. Therefore, both microR-29c and microR-146a levels can be used as biomarkers for the diagnosis of Mycoplasma pneumoniae pneumonia.
背景
我们研究了儿童肺炎支原体肺炎患者血清中 microR-29c 和 microR-146a 的表达变化，分析了它们与炎症因子的关系及其与疾病严重程度的关系，并评估了它们的诊断意义。
方法
56 例肺炎支原体肺炎患儿纳入肺炎支原体肺炎组，37 例健康儿童纳入对照组。采用实时荧光定量逆转录聚合酶链反应检测 microR-29c 和 microR-146a 血清表达水平，酶联免疫吸附试验检测白细胞介素-17、肿瘤坏死因子-α和白细胞介素-1β水平。采用 Pearson 法分析血清 microR-29c 或 microR-146a 表达与炎症因子的相关性。采用受试者工作特征曲线评价血清 microR-29c、microR-146a 及其联合检测对肺炎支原体肺炎的诊断价值。
结果
与健康儿童相比，肺炎支原体肺炎患儿血清 microR-29c 和 microR-146a 水平明显下调，重症患儿下调更为明显。此外，microR-29c 和 microR-146a 与白细胞介素-17、肿瘤坏死因子-α和白细胞介素-1β的表达增加呈负相关。受试者工作特征曲线显示，microR-29c 和 microR-146a 联合检测对肺炎支原体肺炎的诊断效果较好。
结论
儿童肺炎支原体肺炎患者血清 microR-29c 和 microR-146a 表达下调，联合检测 microR-29c 和 microR-146a 可显著提高诊断准确率，因此，microR-29c 和 microR-146a 水平均可作为肺炎支原体肺炎的诊断标志物。
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