Institute of Microbiology, Department of Molecular & Translational Medicine, University of Brescia-Spedali Civili, Brescia, Italy.
Institute of Microbiology & Virology, Department of Biomedical, Surgical & Dental Sciences, University of Milan, Italy.
Future Microbiol. 2019 Oct;14:1281-1292. doi: 10.2217/fmb-2019-0083. Epub 2019 Oct 22.
This study aims to characterize clinical strains of with an extensively drug-resistant phenotype. VITEK 2, Etest method and broth microdilution method for colistin were used. PCR analysis and multilocus sequence typing Pasteur scheme were performed to identify -OXA genes and genetic relatedness, respectively. Whole-genome sequencing analysis was used to characterize three isolates. All the isolates were susceptible only to polymyxins. OXA-23-like gene was the only acquired carbapenemase gene in 88.2% of the isolates. Multilocus sequence typing identified various sequence types: ST2, ST19, ST195, ST577 and ST632. Two new sequence types, namely, ST1279 and ST1280, were detected by whole-genome sequencing. This study showed that carbapenem-resistant isolates causing infections in intensive care units almost exclusively produce OXA-23, underlining their frequent spread in Italy.
本研究旨在对具有广泛耐药表型的 临床分离株进行特征描述。使用 VITEK 2、Etest 法和肉汤微量稀释法检测黏菌素。PCR 分析和多位点序列分型巴斯德方案分别用于鉴定 -OXA 基因和遗传相关性。全基因组测序分析用于对 3 个分离株进行特征描述。所有分离株仅对多黏菌素敏感。OXA-23 样基因是 88.2%的分离株中唯一获得的碳青霉烯酶基因。多位点序列分型确定了各种序列类型：ST2、ST19、ST195、ST577 和 ST632。通过全基因组测序检测到两种新的序列类型，即 ST1279 和 ST1280。本研究表明，引起重症监护病房感染的碳青霉烯类耐药 分离株几乎只产生 OXA-23，这突出了它们在意大利的频繁传播。
