Stephan R N, Conrad P J, Saizawa M, Dean R E, Chaudry I H
Department of Surgery and Physiology, Michigan State University, East Lansing 48824.
J Surg Res. 1988 Jun;44(6):733-9. doi: 10.1016/0022-4804(88)90108-4.
Eicosanoids play a prominent role in trauma. Such mediators of inflammation negatively influence cell-mediated immunity (CMI). There is, however, no information available on the effect of eicosanoids on a critical event in CMI, i.e., antigen-presenting (AP) cell function of macrophages (M luminal diameter), a cellular process responsible for the activation of T and B lymphocytes. The aim of this study, therefore, was to examine the effect of prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) on AP cell function of the peritoneal M luminal diameter. To study this, a T-helper-cell clone, D10.G4.1 was employed. This cell clone proliferates in the presence of Iak (Class II glycoprotein, MAC product) bearing M luminal diameter and specific antigen (conalbumin A) thus directly reflecting the AP capability of the M luminal diameter. Peritoneal M luminal diameter were harvested from B10.BR mice (H2k) and their AP was tested in vitro by incubating varying numbers of M luminal diameter with 2 X 10(4) D10.G4.1 cells/well and conalbumin (400 micrograms/ml) in the presence and absence of different concentrations of PGE2 or TXB2. Cultures were incubated for 72 hr, pulsed with [3H]-thymidine, and harvested. At concentrations of 10, 30, and 100 nM of PGE2, D10.G4.1 proliferations were 38, 35, and 20% of control, respectively (P less than 0.05 compared to control). TXB2 added at the above-mentioned concentrations did not suppress the proliferative response of D10. Thus, PGE2 but not TXB2 has a potent immunosuppressive effect on AP of peritoneal M luminal diameter.(ABSTRACT TRUNCATED AT 250 WORDS)
类花生酸在创伤中起重要作用。这类炎症介质会对细胞介导的免疫（CMI）产生负面影响。然而，关于类花生酸对CMI中一个关键事件，即巨噬细胞（管腔直径M）的抗原呈递（AP）细胞功能的影响，目前尚无相关信息，而巨噬细胞的这一细胞过程负责T和B淋巴细胞的激活。因此，本研究的目的是检测前列腺素E2（PGE2）和血栓素B2（TXB2）对腹膜管腔直径M的AP细胞功能的影响。为研究此问题，采用了T辅助细胞克隆D10.G4.1。该细胞克隆在携带管腔直径M的Iak（II类糖蛋白，MAC产物）和特异性抗原（伴清蛋白A）存在的情况下增殖，从而直接反映管腔直径M的AP能力。从B10.BR小鼠（H2k）获取腹膜管腔直径M，并在有或无不同浓度PGE2或TXB2存在的情况下，将不同数量的管腔直径M与2×10⁴个D10.G4.1细胞/孔以及伴清蛋白（400微克/毫升）一起体外孵育，以检测其AP。培养72小时后，加入[³H] - 胸腺嘧啶核苷脉冲标记，然后收获细胞。在PGE2浓度为10、30和100 nM时，D10.G4.1的增殖分别为对照的38%、35%和20%（与对照相比，P＜0.05）。以上述浓度添加的TXB2并未抑制D10的增殖反应。因此，PGE2而非TXB2对腹膜管腔直径M的AP具有强大的免疫抑制作用。（摘要截选至250字）
