Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang Road West, Haizhu District, Guangzhou, 510300, China.
Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, CAFS Key Laboratory of Aquatic Genomics and Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing, 100141, China.
Sci Data. 2019 Oct 22;6(1):216. doi: 10.1038/s41597-019-0238-8.
Golden pompano (Trachinotus ovatus), a marine fish in the Carangidae family, has a wide geographical distribution and adapts to severe environmental rigours. It is also an economically valuable aquaculture fish. To understand the genetic mechanism of adaption to environmental rigours and improve the production in aquaculture, we assembled its genome. By combination of Illumina and Pacbio reads, the obtained genome sequence is 647.5 Mb with the contig N50 of 1.80 Mb and the scaffold N50 of 5.05 Mb. The assembly covers 98.9% of the estimated genome size (655 Mb). Based on Hi-C data, 99.4% of the assembled bases are anchored into 24 pseudo-chromosomes. The annotation includes 21,915 protein-coding genes, in which 95.7% of 2,586 BUSCO vertebrate conserved genes are complete. This genome is expected to contribute to the comparative analysis of the Carangidae family.
金黄鰤（Trachinotus ovatus），又称金目鲈，为石首鱼科鰤属暖水性近海底层鱼类，广泛分布于印度洋和太平洋西部，适应环境胁迫的能力强，是一种重要的海水增养殖鱼类。为解析金黄鰤适应环境胁迫的遗传机制，提高其养殖生产性能，本研究组装了金黄鰤基因组。研究采用 Illumina 和 Pacbio 测序技术，组装得到基因组大小为 647.5 Mb，contig N50 为 1.80 Mb，scaffold N50 为 5.05 Mb，基因组覆盖度达 98.9%，基于 Hi-C 数据，99.4%的基因组序列锚定到 24 条假染色体上。注释得到 21915 个蛋白编码基因，其中 2586 个脊椎动物保守基因的完整性为 95.7%。金黄鰤基因组的发布将为石首鱼科鱼类比较基因组学研究提供重要资源。
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