Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.
Department of Biotechnology, School of Interdisciplinary and Applied Life Sciences, Central University of Haryana, Jant-Pali, Mahendergarh, Haryana, 123031, India.
Appl Microbiol Biotechnol. 2019 Nov;103(21-22):8763-8784. doi: 10.1007/s00253-019-10108-6. Epub 2019 Oct 22.
Microbial xylanases have gathered great attention due to their biotechnological potential at industrial scale for many processes. A variety of lignocellulosic materials, such as sugarcane bagasse, rice straw, rice bran, wheat straw, wheat bran, corn cob, and ragi bran, are used for xylanase production which also solved the great issue of solid waste management. Both solid-state and submerged fermentation have been used for xylanase production controlled by various physical and nutritional parameters. Majority of xylanases have optimum pH in the range of 4.0-9.0 with optimum temperature at 30-60 °C. For biochemical, molecular studies and also for successful application in industries, purification and characterization of xylanase have been carried out using various appropriate techniques. Cloning and genetic engineering are used for commercial-level production of xylanase, to meet specific economic viability and industrial needs. Microbial xylanases are used in various biotechnological applications like biofuel production, pulp and paper industry, baking and brewing industry, food and feed industry, and deinking of waste paper. This review describes production, characteristics, and biotechnological applications of microbial xylanases.
由于微生物木聚糖酶在工业规模上具有多种生物技术潜力，因此受到了广泛关注。许多木质纤维素材料，如甘蔗渣、稻草、米糠、麦草、麦麸、玉米芯和荞麦麸皮，都被用于生产木聚糖酶，这也解决了大量固体废物管理的问题。固态发酵和液态发酵都被用于生产木聚糖酶，通过各种物理和营养参数进行控制。大多数木聚糖酶的最适 pH 值在 4.0-9.0 之间，最适温度在 30-60°C。为了进行生物化学、分子研究，并成功应用于工业，使用各种合适的技术对木聚糖酶进行了纯化和特性分析。为了满足特定的经济可行性和工业需求，克隆和遗传工程被用于商业规模的木聚糖酶生产。微生物木聚糖酶被广泛应用于各种生物技术应用中，如生物燃料生产、制浆造纸工业、烘焙和酿造工业、食品和饲料工业以及废纸脱墨。本文综述了微生物木聚糖酶的生产、特性和生物技术应用。
