Helseth E, Unsgaard G, Dalen A, Vik R
Institute of Cancer Research, University of Trondheim, Norway.
Cancer Immunol Immunother. 1988;26(3):273-9. doi: 10.1007/BF00199941.
Type beta transforming growth factor (beta-TGF) is a potent regulator of cell growth and differentiation. The human glioblastoma cell line, T-MGI, was growth inhibited by beta-TGF under anchorage independent conditions. The antiproliferative effect of beta-TGF was potentiated to nearly total arrest by low doses of retinoic acid (RA) or tumor necrosis factor (TNF), while epidermal growth factor, platelet-derived growth factor, interleukin-2, and gamma interferon did not have this potentiating effect. The potentiation of the beta-TGF effect by RA and TNF could not be explained by modulation of the epidermal growth factor receptor, the beta-TGF receptor, or the TNF receptor. beta-TGF alone and in combination with RA or TNF were further tested on primary cultures from freshly resected human glioma biopsies (n = 13). There was great individual variation in sensitivity to beta-TGF, RA, or TNF. The astrocytoma and oligodendroglioma cells were inhibited to various degrees by beta-TGF or TNF, while most of the glioblastomas were not sensitive to these agents. Most of the biopsies were stimulated by RA. RA or TNF did not potentiate the growth inhibitory effect of beta-TGF on biopsy cells. We therefore think it unlikely that beta-TGF in combination with RA or TNF will be effective agents in the treatment of gliomas.
β型转化生长因子（β-TGF）是细胞生长和分化的强效调节剂。人胶质母细胞瘤细胞系T-MGI在非贴壁条件下受到β-TGF的生长抑制。低剂量视黄酸（RA）或肿瘤坏死因子（TNF）可增强β-TGF的抗增殖作用，使其几乎完全停滞生长，而表皮生长因子、血小板衍生生长因子、白细胞介素-2和γ干扰素则没有这种增强作用。RA和TNF对β-TGF作用的增强不能通过表皮生长因子受体、β-TGF受体或TNF受体的调节来解释。对来自新鲜切除的人脑胶质瘤活检组织（n = 13）的原代培养物进一步测试了单独的β-TGF以及与RA或TNF联合使用的情况。对β-TGF、RA或TNF的敏感性存在很大的个体差异。星形细胞瘤和少突胶质细胞瘤细胞受到β-TGF或TNF不同程度的抑制，而大多数胶质母细胞瘤对这些药物不敏感。大多数活检组织受到RA的刺激。RA或TNF并未增强β-TGF对活检细胞的生长抑制作用。因此，我们认为β-TGF与RA或TNF联合使用不太可能成为治疗胶质瘤的有效药物。
