HL-60细胞中组蛋白磷酸化的改变与单核细胞分化特异性相关。

Alterations in histone phosphorylation in HL-60 cells specific to monocytic differentiation.

作者信息

DeBord D G, Baxter C S

机构信息

Department of Environmental Health, University of Cincinnati Medical Center, OH 45267-0056.

出版信息

Cancer Lett. 1988 Jul;41(1):69-74. doi: 10.1016/0304-3835(88)90056-0.

DOI:10.1016/0304-3835(88)90056-0

PMID:3164643

Abstract

In order to examine the role of histone phosphorylation in regulation of the pathway of HL-60 cell differentiation, cells were labelled with [32P]phosphoric acid and histones fractionated by two-dimensional polyacrylamide gel electrophoresis. The monocytic inducer 12-O-tetradecanoylphorbol-13-acetate (TPA) was found to specifically stimulate phosphorylation of histone H2B in a concentration-dependent manner. At a concentration of 100 mM, H2B phosphorylation was stimulated 2.3-fold after 4 h. A second monocytic inducer 1,25-dihydroxy-cholecalciferol (100 nM) also induced phosphorylation specifically in histone H2B. In contrast, the granulocytic inducers DMSO (1.5%) or retinoic acid (1 microM) did not increase phosphorylation in any histone species.

摘要

为了研究组蛋白磷酸化在HL-60细胞分化途径调控中的作用，用[32P]磷酸对细胞进行标记，并通过二维聚丙烯酰胺凝胶电泳对组蛋白进行分级分离。发现单核细胞诱导剂12-O-十四酰佛波醇-13-乙酸酯（TPA）以浓度依赖的方式特异性刺激组蛋白H2B的磷酸化。在100 mM的浓度下，4小时后H2B磷酸化被刺激了2.3倍。第二种单核细胞诱导剂1,25-二羟基胆钙化醇（100 nM）也特异性诱导组蛋白H2B的磷酸化。相比之下，粒细胞诱导剂二甲基亚砜（1.5%）或视黄酸（1 microM）不会增加任何组蛋白种类的磷酸化。