Calibasi-Kocal Gizem, Pakdemirli Ahu, Bayrak Serdar, Ozupek Nazli Mert, Sever Tolga, Basbinar Yasemin, Ellidokuz Hulya, Yigitbasi Turkan
Dokuz Eylul University, Institute of Oncology, Department of Basic Oncology, Izmir, Turkey.
J BUON. 2019 Jul-Aug;24(4):1482-1487.
Curcumin is a natural phytopolyphenol compound isolated from the root of turmeric (Curcuma longa) and possesses a wide range of biological properties. The purpose of this study was to evaluate the antiproliferative, wound healing, anti-invasive and anti-migrative effects of curcumin on HCT-116 and LoVo colorectal cancer cell lines.
The antiproliferative activity of 2.5-75 µM curcumin was tested on HCT-116 and LoVo colorectal cell lines and the viability of the cells was tested with WST-1 reagent by using ELISA plate reader at 450 nm. xCELLigence RTCA DP system was used for the detection of anti-invasive and anti-migrative effects of curcumin.
The IC50 of curcumin was 10±0.03 for HCT-116 and 20±0.05 µM for LoVo cell lines. The IC50 of curcumin (10µM for HCT-116 and 20 µM for LoVo) showed anti-metastatic activity on these cell lines.
This study showed that curcumin could be evaluated as a promising anti-cancer agent for human colorectal cancer.
姜黄素是从姜黄（Curcuma longa）根中分离出的一种天然植物多酚化合物，具有广泛的生物学特性。本研究的目的是评估姜黄素对HCT - 116和LoVo结肠癌细胞系的抗增殖、伤口愈合、抗侵袭和抗迁移作用。
用2.5 - 75 μM姜黄素对HCT - 116和LoVo结肠癌细胞系进行抗增殖活性测试，并使用酶标仪在450 nm波长下用WST - 1试剂检测细胞活力。采用xCELLigence RTCA DP系统检测姜黄素的抗侵袭和抗迁移作用。
姜黄素对HCT - 116细胞系的IC50为10±0.03 μM，对LoVo细胞系的IC50为20±0.05 μM。姜黄素的IC50（HCT - 116为10 μM，LoVo为20 μM）对这些细胞系显示出抗转移活性。
本研究表明，姜黄素可被评估为一种有前景的人类结直肠癌抗癌药物。
