Instituto de Microbiologia Paulo de Goes, Universidade Federal Do Rio De Janeiro, Rio De Janeiro, Brazil.
Biofouling. 2019 Sep;35(8):938-944. doi: 10.1080/08927014.2019.1678592. Epub 2019 Oct 24.
(group B , GBS) is a major pathogen in humans and animals. Pili and biofilm may be important virulence factors in this bacterial species. Here, biofilm production and the distribution of pilus variants among 134 GBS isolates from human and animal sources were evaluated. Biofilm production was significantly enhanced in 1% glucose-supplemented medium ( < 0.05). Using this medium, most GBS strains were strong biofilm producers. Biomass was mainly composed of proteins, followed by extracellular DNA, while polysaccharides represented a minor portion. All GBS strains presented at least one pilus variant. PI-2a was the most common among human GBS while PI-2b was the most common among animal isolates. Human GBS harboring PI-2b and animal GBS harboring PI-2a presented significantly reduced biofilm production ( = 0.0033). In conclusion, strong biofilm production seems to be a common characteristic in GBS, and association of the clinical source with the pilus variant may be crucial for this.
（GBS）是人类和动物的主要病原体。菌毛和生物膜可能是该细菌物种的重要毒力因子。在此，评估了来自人和动物来源的 134 株 GBS 分离株中生物膜的产生和菌毛变体的分布。在 1%葡萄糖补充培养基中（ < 0.05），生物膜的产生显著增强。使用这种培养基，大多数 GBS 菌株都是强生物膜生产者。生物量主要由蛋白质组成，其次是细胞外 DNA，而多糖则占很小一部分。所有 GBS 菌株都至少存在一种菌毛变体。PI-2a 在人源 GBS 中最为常见，而 PI-2b 在动物分离株中最为常见。携带 PI-2b 的人源 GBS 和携带 PI-2a 的动物 GBS 的生物膜产生明显减少（ = 0.0033）。总之，强生物膜的产生似乎是 GBS 的一个共同特征，而临床来源与菌毛变体的关联可能对此至关重要。
