Liu Nuan, Cui Wenbo, Chen Mingyue, Zhang Xuelian, Song Xiaoyue, Pan Chuanying
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
Shaanxi Provincial Engineering and Technology Research Center of Cashmere Goats, Yulin University, Yulin, Shaanxi, China.
Anim Biotechnol. 2021 Apr;32(2):213-218. doi: 10.1080/10495398.2019.1677682. Epub 2019 Oct 24.
The scribble cell polarity complex component (LLGL1) is part of the cytoskeletal network and is involved in maintaining cell polarity and epithelial integrity. Based on the whole-genome sequencing analysis in goat, gene is suggested as a putative important candidate gene affecting litter size in Shaanbei White Cashmere Goats (SBWC). Therefore, the objective of this study was to uncover the possible novel insertion/deletion (Indel) variant in goat gene and to evaluate its association with litter size of SBWC ( = 827). Using the PCR detection and DNA sequencing, the 21-bp indel in the upstream of was firstly founded and two genotypes were identified: II (insertion/insertion) and ID (insertion/deletion), respectively. Association analyses revealed that the 21-bp indel was significantly correlated with litter size ( = 0.017). Notably, the individuals with II genotype were significantly greater than that of the genotype ID, and the 'I' allele was dominant. Additionally, the remarkable influence of the indel on traits might be related to the change of DEAF-1-related (NUDR) binding site through bioinformatics analysis. Briefly, the 21-bp indel within the goat gene could be an effective DNA molecular marker and provide valuable theoretical basis for marker-assisted selection (MAS) in goat industry.
scribble细胞极性复合体组分（LLGL1）是细胞骨架网络的一部分，参与维持细胞极性和上皮完整性。基于山羊全基因组测序分析，该基因被认为是影响陕北白绒山羊（SBWC）产羔数的一个重要候选基因。因此，本研究的目的是揭示山羊该基因可能存在的新型插入/缺失（Indel）变异，并评估其与SBWC产羔数（n = 827）的关联性。通过PCR检测和DNA测序，首次在该基因上游发现了21bp的Indel，并鉴定出两种基因型：II（插入/插入）和ID（插入/缺失）。关联分析表明，该21bp的Indel与产羔数显著相关（P = 0.017）。值得注意的是，II基因型个体的产羔数显著高于ID基因型，且“I”等位基因呈显性。此外，通过生物信息学分析发现，该Indel对性状的显著影响可能与DEAF-1相关（NUDR）结合位点的变化有关。简而言之，山羊该基因内的21bp Indel可能是一种有效的DNA分子标记，为山羊产业的标记辅助选择（MAS）提供有价值的理论依据。
