Department of Andrology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.
Department of Andrology, Beijing's Capital Medical University Traditional Chinese Medicine Hospital, Beijing, 100010, China.
Chin J Integr Med. 2019 Sep;25(9):671-676. doi: 10.1007/s11655-019-3203-7. Epub 2019 Oct 24.
To evaluate the effects of Xiaojin Pill () in the treatment of Peyronie's disease (PD) in a rat model.
Twenty-four male Sprague-Dawley rats were randomly divided into four groups with 6 in each: sham operation, PD model, vehicle control and Xiaojin Pill groups. The rats in the sham operation group received penile tunica albsginea (TA) injection with 50 μL vehicle, while the rats in the other 3 groups received 50 μL penile TA injection of 50 μg transforming growth factor (TGF)-β1. Forty-two days after the injection, rats in the vehicle control and Xiaojin Pill groups received 0.5 mL water and Xiaojin Pill solution (107 mg/kg of body weight), respectively by gavage for 28 days, while those in the sham operation and PD model groups did not receive any intervention. After intervention, the expressions of matrix metalloproteinase 2/9 (MMP2/9), nitric oxidesynthase (NOS), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured.
Rats in the PD model and vehicle control groups presented obvious fibrosis in corpus cavernosum (CC) and demonstrated a significantly increased expressions of MMP2 and MMP9 in the CC compared with the sham operation group (all P<0.01). In contrast, the expressions of MMP2 and MMP9 in the Xiaojin Pill group were significantly down-regulated (both P<0.01). In addition, the levels of NOS and MDA in CC were significantly increased while the activity of SOD was decreased in the PD model and vehicle control groups compared with the sham operation group (all P<0.01). After Xiaojin Pill treatment, the levels of MDA, NOS and SOD appeared to be corrected (all P<0.01).
Xiaojin Pill could reduce fibrosis in the CC by decreasing the expressions of MMPs, NOS and MDA, and by increasing the activity of SOD. Therefore, Xiaojin Pill might be a therapeutic option for PD.
评估小金丸（）治疗大鼠模型中 Peyronie 病（PD）的疗效。
将 24 只雄性 Sprague-Dawley 大鼠随机分为 4 组，每组 6 只：假手术组、PD 模型组、对照组和小金丸组。假手术组大鼠阴茎白膜（TA）注射 50 μL 载体，其余 3 组大鼠 TA 注射 50 μL 50 μg 转化生长因子（TGF）-β1。注射后 42 天，对照组和小金丸组大鼠分别灌胃 0.5 mL 水和小金丸溶液（107 mg/kg 体重），28 天；假手术组和 PD 模型组大鼠未进行任何干预。干预后，测量基质金属蛋白酶 2/9（MMP2/9）、一氧化氮合酶（NOS）、超氧化物歧化酶（SOD）和丙二醛（MDA）的表达。
PD 模型组和对照组大鼠海绵体（CC）明显纤维化，CC 中 MMP2 和 MMP9 的表达明显高于假手术组（均 P<0.01）。相反，小金丸组 MMP2 和 MMP9 的表达明显下调（均 P<0.01）。此外，与假手术组相比，PD 模型组和对照组 CC 中 NOS 和 MDA 水平明显升高，SOD 活性降低（均 P<0.01）。小金丸治疗后，MDA、NOS 和 SOD 水平似乎得到纠正（均 P<0.01）。
小金丸可通过降低 MMPs、NOS 和 MDA 的表达，增加 SOD 的活性，减少 CC 纤维化，可能是 PD 的一种治疗选择。
