Wachtel S, Nakamura D, Wachtel G, Felton W, Kent M, Jaswaney V
Center for Reproductive Biology, Collierville, Tennessee.
Fertil Steril. 1988 Aug;50(2):355-60.
H-Y antigen defined by antibody from male-sensitized female mice has been reported in male embryos of mouse, rat, cattle, goat, pig, and sheep. We now describe the use of monoclonal H-Y antibodies in identification of male and female during embryo transfer in cattle. Monoclonal H-Y antibodies were applied with fluorescein-isothiocyanate-conjugated goat antimouse immunoglobulin (Ig), and the embryos were scored for fluorescence under ultraviolet light. In alternative trials with 149 embryos, the method could be applied with 73% to 82% efficiency. Pregnancy rates for treated embryos were at least as high as those expected for untreated embryos. The calves that developed from the treated embryos are normal and growing.
由雄性致敏雌性小鼠产生的抗体所定义的H-Y抗原已在小鼠、大鼠、牛、山羊、猪和绵羊的雄性胚胎中被报道。我们现在描述单克隆H-Y抗体在牛胚胎移植过程中用于鉴别雌雄胚胎的应用。将单克隆H-Y抗体与异硫氰酸荧光素偶联的山羊抗小鼠免疫球蛋白（Ig）一起应用，然后在紫外线下对胚胎的荧光进行评分。在对149个胚胎进行的其他试验中，该方法的应用效率为73%至82%。经处理胚胎的妊娠率至少与未处理胚胎预期的妊娠率一样高。由经处理胚胎发育而来的小牛正常生长。
