Hings I, Billingham R E
J Reprod Immunol. 1984 Dec;6(6):345-52. doi: 10.1016/0165-0378(84)90044-5.
C57BL/6J female mice were splenectomized and then sensitized to the H-Y antigen by means of grafts of H-Y incompatible skin and injections of spleen cells from syngeneic males. When subsequently mated to syngeneic males the secondary sex ratio of progeny born was significantly greater than that of controls. However, the effect was manifest only among the progeny of later litters (i.e., 3, 4, 5) suggesting that repeated exposure to the H-Y antigen during the course of pregnancy was essential to bring about demonstrable immunoregulatory changes.
C57BL/6J雌性小鼠接受脾切除手术，然后通过移植H-Y不相容皮肤和注射同基因雄性小鼠的脾细胞，使其对H-Y抗原致敏。随后，当这些小鼠与同基因雄性小鼠交配时，所产后代的第二性别比例显著高于对照组。然而，这种效应仅在后期产仔（即第3、4、5窝）的后代中表现出来，这表明在怀孕期间反复接触H-Y抗原对于引起明显的免疫调节变化至关重要。
