Hu Xin, Xing Yishen, Ren Ling, Wang Yahui, Li Qian, Fu Xing, Yang Qiyuan, Xu Lingyang, Willems Luc, Li Junya, Zhang Lupei
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Molecular and Cellular Biology, Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, Belgium.
Animals (Basel). 2019 Oct 24;9(11):859. doi: 10.3390/ani9110859.
MicroRNAs modulate a variety of cellular events, including skeletal muscle development, but the molecular basis of their functions in fetal bovine skeletal muscle development is poorly understood. In this study, we report that bta-miR-24-3p promotes the myogenic differentiation of fetal bovine PDGFRα progenitor cells. The expression of bta-miR-24-3p increased during myogenic differentiation. Overexpression of bta-miR-24-3p significantly promoted myogenic differentiation, but inhibited proliferation. A dual-luciferase assay identified as a direct target of bta-miR-24-3p. Similarly, knocking down by RNA interference also significantly inhibited proliferation and promoted the differentiation of bovine PDGFRα progenitor cells. Thus, our study provides a mechanism in which bta-miR-24-3p regulates myogenesis by inhibiting expression.
微小RNA调节多种细胞活动，包括骨骼肌发育，但其在胎牛骨骼肌发育中功能的分子基础仍知之甚少。在本研究中，我们报道bta-miR-24-3p促进胎牛血小板衍生生长因子受体α（PDGFRα）祖细胞的成肌分化。bta-miR-24-3p的表达在成肌分化过程中增加。bta-miR-24-3p的过表达显著促进成肌分化，但抑制增殖。双荧光素酶测定确定[此处原文缺失具体基因名]为bta-miR-24-3p的直接靶标。同样，通过RNA干扰敲低[此处原文缺失具体基因名]也显著抑制增殖并促进牛PDGFRα祖细胞的分化。因此，我们的研究提供了一种机制，即bta-miR-24-3p通过抑制[此处原文缺失具体基因名]的表达来调节肌发生。
