Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, College of Life Sciences, Northwest University, Xi'an 710069, China.
School of Chemical Engineering, Northwest University, Xi'an 710069, China.
Int J Mol Sci. 2019 Oct 24;20(21):5285. doi: 10.3390/ijms20215285.
Many plant endophytes produce mycotoxins, but how host genetic variation influences endophyte colonization and mycotoxin production under natural conditions is poorly understood. This interaction has not been fully considered in many previous studies which used controlled experiments with agronomic or model plant species. Here, we investigated this interaction in a naturally occurring forb (a locoweed species) , its symbiotic endophyte , and the mycotoxin swainsonine. Host genetic variation was characterized by microsatellite markers. Endophyte infection rate and swainsonine levels were determined by PCR and HPLC, respectively. Genetic markers defined two distinct host populations and revealed that host genetics were significantly correlated with geographical location, elevation, and precipitation. As the host diverged, symbiotic interactions were reduced or failed to produce detectable swainsonine in one host population. Host genotype and precipitation had a significant impact in shaping swainsonine production at the population level. This study highlights the effect of host genotype in influencing this interaction in locoweeds.
许多植物内生真菌会产生霉菌毒素，但宿主遗传变异如何影响内生真菌定殖和自然条件下霉菌毒素的产生还知之甚少。在许多以前的研究中，由于使用了农业或模式植物物种的控制实验，因此没有充分考虑到这种相互作用。在这里，我们在一种自然发生的野草（一种疯草）、其共生内生真菌和霉菌毒素 swainsonine 中研究了这种相互作用。通过微卫星标记来描述宿主遗传变异。通过 PCR 和 HPLC 分别确定内生菌感染率和 swainsonine 水平。遗传标记定义了两个不同的宿主种群，并表明宿主遗传学与地理位置、海拔和降水量显著相关。随着宿主的分化，共生关系减少或未能在一个宿主种群中产生可检测到的 swainsonine。宿主基因型和降水量对种群水平上 swainsonine 产生有显著影响。本研究强调了宿主基因型在影响疯草中这种相互作用的作用。
