Wang Jieshu, Li Bo, Zhao Kun, Su Xinyou
Department of Gastroenterology, Binzhou Center Hospital, Binzhou, 251700 Shandong People's Republic of China.
2Department of Oncology, Jinan Central Hospital, No. 105, Jiefang Road, Lixia District, Jinan, 250013 Shandong People's Republic of China.
3 Biotech. 2019 Nov;9(11):384. doi: 10.1007/s13205-019-1915-1. Epub 2019 Oct 4.
The present study was aimed to investigate the effect of 2-amino-4-(1-piperidine) pyridine on migration and invasion of colon cancer cells. Treatment of colon cancer cells with 2-amino-4-(1-piperidine) pyridine reduced viability in concentration-based manner. The migration potential of HCT116 and HT29 cells was also suppressed on treatment with 2-amino-4-(1-piperidine) pyridine. In HCT116 and HT29 cells, apoptotic cell proportion was increased significantly by 2-amino-4-(1-piperidine) pyridine treatment. The expression of EMT and Vimentin in HCT116 and HT29 cells was reduced markedly on treatment with 2-amino-4-(1-piperidine) pyridine. The expression of E-cadherin was increased in HCT116 and HT29 cells by 2-amino-4-(1-piperidine) pyridine treatment. Treatment with 2-amino-4-(1-piperidine) pyridine reduced the expression of FOXA2 in HCT116 and HT29 cells. The 2-amino-4-(1-piperidine) pyridine treatment reduced growth of tumor in vivo in mice model. In summary, 2-amino-4-(1-piperidine) pyridine treatment inhibits colon cancer cell proliferation through down-regulation of FOXA2 expression. Therefore, 2-amino-4-(1-piperidine) pyridine can be used for the treatment of colon cancer.
本研究旨在探讨2-氨基-4-(1-哌啶基)吡啶对结肠癌细胞迁移和侵袭的影响。用2-氨基-4-(1-哌啶基)吡啶处理结肠癌细胞会以浓度依赖的方式降低细胞活力。用2-氨基-4-(1-哌啶基)吡啶处理后，HCT116和HT29细胞的迁移能力也受到抑制。在HCT116和HT29细胞中，用2-氨基-4-(1-哌啶基)吡啶处理可显著增加凋亡细胞比例。用2-氨基-4-(1-哌啶基)吡啶处理后，HCT116和HT29细胞中EMT和波形蛋白的表达明显降低。用2-氨基-4-(1-哌啶基)吡啶处理可使HCT116和HT29细胞中E-钙黏蛋白的表达增加。用2-氨基-4-(1-哌啶基)吡啶处理可降低HCT116和HT29细胞中FOXA2的表达。在小鼠模型中，用2-氨基-4-(1-哌啶基)吡啶处理可抑制体内肿瘤生长。综上所述，2-氨基-4-(1-哌啶基)吡啶处理通过下调FOXA2表达抑制结肠癌细胞增殖。因此，2-氨基-4-(1-哌啶基)吡啶可用于治疗结肠癌。
