循环血浆 microRNA 特征用于宫颈癌的诊断。

Circulating plasma microRNA signature for the diagnosis of cervical cancer.

机构信息

Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu, China.

Department of Pathology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

出版信息

Cancer Biomark. 2019;26(4):491-500. doi: 10.3233/CBM-190256.

DOI:10.3233/CBM-190256

PMID:31658043

Abstract

OBJECTIVE

To determine the utility of plasma microRNAs (miRNAs) as biomarkers in cervical cancer (CC).

METHODS

Some studies were conducted about the specific expression of plasma miRNAs in the diagnosis of CC. Plasma samples of 97 CC patients and 87 normal controls (NCs) were used to identify dysregulation of miRNAs in the training, testing, and external validation phases. Receiver operating characteristic (ROC) curves and area under the ROC curve (AUC) were used to evaluate the sensitivity and specificity of identified individual miRNAs and miRNA panels for the diagnosis of CC. Expression levels of specific miRNAs were also examined in plasma exosomes and tissue samples of CC patients.

RESULTS

Four plasma miRNAs (miR-146a-5p, miR-151a-3p, miR-2110 and miR-21-5p) which showed up-regulation were identified and validated in CC patients. A panel of the four miRNAs were constructed as potential diagnostic markers for CC. The AUCs of the panel of these four-miRNAs for the training, testing, and external validation phases were 0.911, 0.774, and 0.786, respectively. miR-146a-5p and miR-21-5p levels were all up-regulated in CC tissue specimens, whereas miR-146a-5p, miR-151a-3p, and miR-2110 levels were up-regulated in plasma exosomes.

CONCLUSION

The signature of the four-miRNAs identified in peripheral plasma is a promising novel biomarker for the diagnosis of CC.

摘要

目的

确定血浆 microRNAs（miRNAs）作为宫颈癌（CC）生物标志物的效用。

方法

对血浆 miRNAs 在 CC 诊断中的特异性表达进行了一些研究。在训练、测试和外部验证阶段，使用 97 例 CC 患者和 87 例正常对照（NC）的血浆样本，鉴定 miRNA 的失调情况。接收者操作特征（ROC）曲线和 ROC 曲线下面积（AUC）用于评估鉴定的个体 miRNA 和 miRNA 组合用于 CC 诊断的灵敏度和特异性。还检查了 CC 患者血浆外泌体和组织样本中特定 miRNA 的表达水平。

结果

在 CC 患者中鉴定并验证了四个上调的血浆 miRNA（miR-146a-5p、miR-151a-3p、miR-2110 和 miR-21-5p）。构建了一个由这四个 miRNA 组成的 panel，作为 CC 的潜在诊断标志物。该 panel 在训练、测试和外部验证阶段的 AUC 分别为 0.911、0.774 和 0.786。CC 组织标本中 miR-146a-5p 和 miR-21-5p 水平均上调，而 miR-146a-5p、miR-151a-3p 和 miR-2110 水平在外泌体中上调。