一种新型微小RNA miR-4433a-3p作为肺腺癌潜在的诊断生物标志物。
A novel microRNA miR-4433a-3p as a potential diagnostic biomarker for lung adenocarcinoma.
作者信息
Sun Zhixiao, Sun Jian, Hu Hang, Han Shuhua, Ma Panpan, Zuo Bingqing, Wang Zheng, Liu Zhongxiang
机构信息
Department of Pulmonary and Critical Care Medicine, The Yancheng Clinical College of Xuzhou Medical University, The First People's Hospital of Yancheng, China.
Department of Central Laboratory, The Yancheng Clinical College of Xuzhou Medical University, The First People's Hospital of Yancheng, China.
出版信息
Heliyon. 2024 May 4;10(9):e30646. doi: 10.1016/j.heliyon.2024.e30646. eCollection 2024 May 15.
BACKGROUND
Lung adenocarcinoma is one of the leading causes of cancer-related deaths because of the lack of early specific clinical indicators. MicroRNAs (miRNAs) have become the focus in lung cancer diagnosis. Further studies are required to explore miRNA expression in the serum of lung adenocarcinoma patients and their correlation with therapy and analyse specific messenger RNA targets to improve the specificity and sensitivity of early diagnosis.
METHODS
The Toray 3D-Gene miRNA array was used to compare the expression levels of various miRNAs in the sera of patients with lung adenocarcinoma and healthy volunteers. Highly expressed miRNAs were selected for further analysis. To verify the screening results, serum and pleural fluid samples were analysed using qRT-PCR. Serum levels of the miRNAs and their correlation with the clinical information of patients with lung adenocarcinoma were analysed. The functions of miRNAs were further analysed using the Kyoto Encyclopedia of Gene and Genomes and Gene Ontology databases.
RESULTS
Microarray analysis identified 60 and 50 miRNAs with upregulated and downregulated expressions, respectively, in the serum of patients with lung adenocarcinoma compared to those in healthy individuals. Using qRT-qPCR to detection of miRNAs expression in the serum or pleural effusion of patients with early and advanced lung adenocarcinoma, we found that miR-4433a-3p could be used as a diagnostic marker and therapeutic evaluation indicator for lung adenocarcinoma. Serum of miR-4433a-3p levels significantly correlated with the clinical stage. miR-4433a-3p may be more suitable than other tumour markers for the early diagnosis and evaluation of therapeutic effects in lung adenocarcinoma. miR-4433a-3p may affect tumour growth and metastasis by acting on target genes ( and others) and regulating tumour-related signalling pathways (MAPK signal pathway, Ras signalling pathway and others).
CONCLUSION
miR-4433a-3p may serve as a biomarker for the early diagnosis of lung adenocarcinoma and monitoring of therapeutic effects.
背景
由于缺乏早期特异性临床指标，肺腺癌是癌症相关死亡的主要原因之一。微小RNA（miRNA）已成为肺癌诊断的焦点。需要进一步研究以探索肺腺癌患者血清中miRNA的表达及其与治疗的相关性，并分析特定的信使RNA靶点，以提高早期诊断的特异性和敏感性。
方法
使用东丽3D-Gene miRNA芯片比较肺腺癌患者和健康志愿者血清中各种miRNA的表达水平。选择高表达的miRNA进行进一步分析。为了验证筛选结果，使用qRT-PCR分析血清和胸水样本。分析miRNA的血清水平及其与肺腺癌患者临床信息的相关性。使用京都基因与基因组百科全书和基因本体数据库进一步分析miRNA的功能。
结果
微阵列分析发现，与健康个体相比，肺腺癌患者血清中分别有60种和50种miRNA表达上调和下调。通过qRT-qPCR检测早期和晚期肺腺癌患者血清或胸水中miRNA的表达，我们发现miR-4433a-3p可作为肺腺癌的诊断标志物和治疗评估指标。miR-4433a-3p的血清水平与临床分期显著相关。miR-4433a-3p可能比其他肿瘤标志物更适合用于肺腺癌的早期诊断和治疗效果评估。miR-4433a-3p可能通过作用于靶基因（及其他基因）并调节肿瘤相关信号通路（MAPK信号通路、Ras信号通路等）来影响肿瘤生长和转移。
结论
miR-4433a-3p可作为肺腺癌早期诊断和治疗效果监测的生物标志物。
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