Department of Obstetrics and Gynecology, Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Department of Gynecology Oncology, First People's Hospital of Chenzhou, Chenzhou, Hunan, China.
Cancer Biomark. 2019;26(4):481-489. doi: 10.3233/CBM-190328.
The aim of the present study was to investigate kinesin family member 7 (KIF7) expression in epithelial ovarian cancer tissues (paraffin-embedded tissues and fresh) and to explore its expression, association with clinicopathological parameters and prognostic value in patients with epithelial ovarian cancer. A total of 113 paraffin-embedded tumor tissues of epithelial ovarian cancer patients diagnosed and operated at the memorial hospital of Sun Yat-sen University Between December 2009 and March 2017 and 41 paratumor tissues were collected for the present study and were assessed for KIF7 expression using immunohistochemistry. Furthermore, 22 fresh epithelial ovarian cancer tissues and their matched paratumor tissues were collected from the Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, between August 2013 and March 2019 and subjected to reverse-transcription quantitative PCR analysis to detect the mRNA expression of KIF7. The expression of KIF7 was lower in cancer tissues than in paratumor tissues, and KIF7 expression was associated with recurrence-free survival and overall survival in epithelial ovarian cancer patients. Furthermore, multivariate logistic regression analysis indicated that low KIF7 expression was an independent predictor of poor survival in patients with epithelial ovarian cancer. In conclusion, KIF7 has a tumor suppressor role in epithelial ovarian cancer and is a useful independent prognostic predictor. It may hold important value for the clinical diagnosis and treatment of epithelial ovarian cancer.
本研究旨在探讨驱动蛋白家族成员 7（KIF7）在卵巢上皮性癌组织（石蜡包埋组织和新鲜组织）中的表达，并探讨其在卵巢上皮性癌患者中的表达、与临床病理参数的关系及其预后价值。本研究共收集了中山大学孙逸仙纪念医院 2009 年 12 月至 2017 年 3 月诊断和手术的 113 例卵巢上皮性癌石蜡包埋肿瘤组织和 41 例癌旁组织，采用免疫组织化学法评估 KIF7 的表达。此外，本研究还收集了 2013 年 8 月至 2019 年 3 月南方医科大学中西医结合医院的 22 例新鲜卵巢上皮性癌组织及其配对的癌旁组织，采用逆转录定量 PCR 分析检测 KIF7 的 mRNA 表达。结果显示，癌组织中 KIF7 的表达低于癌旁组织，KIF7 的表达与卵巢上皮性癌患者的无复发生存和总生存相关。此外，多因素 logistic 回归分析表明，KIF7 低表达是卵巢上皮性癌患者不良生存的独立预测因素。综上所述，KIF7 在卵巢上皮性癌中发挥抑癌作用，是一种有用的独立预后预测因子。它可能对卵巢上皮性癌的临床诊断和治疗具有重要价值。
