Department of Biology, Duke University, Durham, NC, USA.
Department of Orthopaedic Surgery, Duke University School of Medicine, Durham, NC, USA.
Connect Tissue Res. 2020 May-Jul;61(3-4):322-337. doi: 10.1080/03008207.2019.1680656. Epub 2019 Oct 29.
: Meniscal injury and loss of meniscus tissue lead to osteoarthritis development. Therefore, novel biologic strategies are needed to enhance meniscus tissue repair. The purpose of this study was to identify a favorable culture medium for both bone marrow-derived mesenchymal stem cells (MSCs) and meniscal tissue, and to establish a novel meniscus tissue defect model that could be utilized for screening of biologics to promote meniscus repair.: In parallel, we analyzed the biochemical properties of MSC - seeded meniscus-derived matrix (MDM) scaffolds and meniscus repair model explants cultured in different combinations of serum, dexamethasone (Dex), and TGF-β. Next, we combined meniscus tissue and MSC-seeded MDM scaffolds into a novel meniscus tissue defect model to evaluate the effects of chondrogenic and meniscal media on the tissue biochemical properties and repair strength.: Serum-free medium containing TGF-β and Dex was the most promising formulation for experiments with MSC-seeded scaffolds, whereas serum-containing medium was the most effective for meniscus tissue composition and integrative repair. When meniscus tissue and MSC-seeded MDM scaffolds were combined into a defect model, the chondrogenic medium (serum-free with TGF-β and Dex) enhanced the production of proteoglycans and promoted integrative repair of meniscus tissue. As well, cross-linked scaffolds improved repair over the MDM slurry.: The meniscal tissue defect model established in this paper can be used to perform screening to identify and optimize biological treatments to enhance meniscus tissue repair prior to conducting preclinical animal studies.
: 半月板损伤和半月板组织丢失会导致骨关节炎的发展。因此，需要新的生物策略来增强半月板组织修复。本研究的目的是确定一种有利于骨髓间充质干细胞（MSCs）和半月板组织的培养介质，并建立一种新的半月板组织缺损模型，可用于筛选促进半月板修复的生物制剂。：同时，我们分析了在不同血清、地塞米松（Dex）和 TGF-β组合中培养的 MSC 种植的半月板衍生基质（MDM）支架和半月板修复模型外植体的生化特性。接下来，我们将半月板组织和 MSC 种植的 MDM 支架结合成一种新的半月板组织缺损模型，以评估软骨和成半月板培养基对组织生化特性和修复强度的影响。：含 TGF-β 和 Dex 的无血清培养基是 MSC 种植支架实验最有前途的配方，而含血清的培养基最有利于半月板组织成分和整体修复。当半月板组织和 MSC 种植的 MDM 支架结合成缺损模型时，软骨培养基（无血清，含 TGF-β 和 Dex）可增加蛋白聚糖的产生并促进半月板组织的整体修复。交联支架可改善 MDM 浆料的修复效果。：本文建立的半月板组织缺损模型可用于进行筛选，以识别和优化生物治疗方法，以增强半月板组织修复，然后再进行临床前动物研究。
