Correia Hudson H V, Lima Laritza F, Sousa Francisca Geovania C, Ferreira Anna Clara A, Cadenas Jesus, Paes Victor M, Alves Benner G, Shikanov Ariella, Figueiredo José Ricardo
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, Brazil.
Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Reprod Domest Anim. 2020 Jan;55(1):105-109. doi: 10.1111/rda.13582. Epub 2019 Nov 27.
The present study aimed to evaluate the effect of three culture systems on caprine primordial follicle activation in vitro: follicles cultured either in the isolated form within alginate (Isolated follicles + Alginate treatment), or enclosed in ovarian tissue (in situ), with or without alginate (Fragment + Alginate, and Fragment alone treatments, respectively). After culture, the Isolated follicles + Alginate treatment presented a percentage of morphologically normal follicles (MNF) similar to both the non-cultured control and the Fragment Alone treatments. Nevertheless, Fragment + Alginate treatment showed a significant reduction in the number of MNF when compared to the other treatments. Regarding follicle development, our results showed that regardless of the alginate, the presence of ovarian tissue limited primordial follicle activation during in vitro culture. Remarkably, the Isolated primordial follicle + Alginate treatment was the only one that significantly promoted follicle activation and increased both follicle and oocyte diameters during IVFC, pointing out a higher cell proliferation. In conclusion, the presence of ovarian tissue with or without alginate limited follicle development (activation) after culture. Nevertheless, when primordial follicles were isolated and encapsulated in alginate they presented suitable survival rates, higher rates of follicle activation and continued to grow throughout the culture period.
卵泡分别以分离形式培养于藻酸盐中（分离卵泡 + 藻酸盐处理），或包埋于卵巢组织中（原位），有无藻酸盐处理（分别为碎片 + 藻酸盐处理和单独碎片处理）。培养后，分离卵泡 + 藻酸盐处理组的形态正常卵泡（MNF）百分比与未培养对照组及单独碎片处理组相似。然而，与其他处理相比，碎片 + 藻酸盐处理组的MNF数量显著减少。关于卵泡发育，我们的结果表明，无论有无藻酸盐，卵巢组织的存在都会限制体外培养期间原始卵泡的激活。值得注意的是，分离原始卵泡 + 藻酸盐处理是唯一在体外卵泡培养（IVFC）过程中显著促进卵泡激活并增加卵泡和卵母细胞直径的处理，表明细胞增殖更高。总之，有无藻酸盐的卵巢组织在培养后都会限制卵泡发育（激活）。然而，当原始卵泡被分离并包埋于藻酸盐中时，它们具有合适的存活率、更高的卵泡激活率，并在整个培养期间持续生长。
