Kang Gaowa, Mishyna Maryia, Appiah Kwame Sarpong, Yamada Masaaki, Takano Akihito, Prokhorov Valery, Fujii Yoshiharu
Department of Biological Production Science, United Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
School of Food Science and Biotechnology, Zhejiang Gongshang University, 18, Xuezheng Street Hangzhou, Zhejiang 310018, China.
Plants (Basel). 2019 Oct 28;8(11):457. doi: 10.3390/plants8110457.
One hundred and thirty-nine medicinal plant species were screened for their allelopathic activity through volatile emissions using as a test plant. Volatile emissions from the leaves of star anise () showed the highest inhibition (100%) on the radicle and hypocotyl growth. Using headspace gas collection and gas chromatography-mass spectrometry (GC-MS), seven major volatile compounds from the leaves of star anise, including α-pinene, β-pinene, camphene, 1,8-cineole, D-limonene, camphor, and L-fenchone were detected. To determine volatile compounds that may contribute to the inhibitory activity of star anise, the allelopathic potential of individual volatiles from star anise was evaluated using the cotton swab bioassay. The EC was calculated for each of the seven identified compounds. L-fenchone showed the strongest growth inhibitory activity (EC is 1.0 ng/cm for radicle and hypocotyl growth of lettuce), followed by 1,8-cineole, and camphene. This is the first report that L-fenchone could be an important volatile allelochemical from the leaves of star anise. From the actual concentration of each volatile compound in headspace and EC value, we concluded that the four volatile compounds, including L-fenchone, 1,8-cineole, β-pinene, and camphene are the most important contributors to the volatile allelopathy of star anise.
以 作为受试植物，通过挥发性气体排放对139种药用植物进行化感活性筛选。八角茴香（）叶片的挥发性气体排放对胚根和下胚轴生长的抑制作用最强（100%）。采用顶空气体采集和气相色谱 - 质谱联用（GC - MS）技术，从八角茴香叶片中检测出7种主要挥发性化合物，包括α - 蒎烯、β - 蒎烯、莰烯、1,8 - 桉叶素、D - 柠檬烯、樟脑和L - 小茴香酮。为确定可能对八角茴香抑制活性有贡献的挥发性化合物，采用棉签生物测定法评估了八角茴香各挥发性成分的化感潜力。计算了7种已鉴定化合物各自的半数有效浓度（EC）。L - 小茴香酮表现出最强的生长抑制活性（生菜胚根和下胚轴生长的EC为1.0 ng/cm），其次是1,8 - 桉叶素和莰烯。这是首次报道L - 小茴香酮可能是八角茴香叶片中一种重要的挥发性化感物质。根据顶空中各挥发性化合物的实际浓度和EC值，我们得出结论，L - 小茴香酮、1,8 - 桉叶素、β - 蒎烯和莰烯这4种挥发性化合物是八角茴香气化感作用的最重要贡献者。
