Sekine Takayuki, Appiah Kwame Sarpong, Azizi Majid, Fujii Yoshiharu
Miyagi Prefectural Agriculture and Horticulture Research Centre, 1, Higashi-kongouji, Kawakami, Takadate, Natori, Miyagi 981-1243, Japan.
Department of Biological Production Science, United Graduate School of Agriculture, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
Plants (Basel). 2020 Feb 18;9(2):264. doi: 10.3390/plants9020264.
The inhibitory activities of the leachates and volatiles from 53 plant species (spices and herbs) were evaluated against lettuce ( "Great Lakes 366") seedling growth using the sandwich and dish pack methods, respectively. With the sandwich method, parsley () showed the strongest inhibitory effect on lettuce radicle growth (77%), followed by tarragon () (72%). However, caraway (), dill () (seed), laurel (), rosemary (), and sage () were the most inhibitory species (100% inhibition of lettuce radicle and hypocotyl growth inhibition at all distance wells) in the dish pack method. Cardamom () and thyme () also showed strong inhibitory activity (100% for radicle and hypocotyl growth inhibition at all 41 and 58 mm distance wells). The headspace sampling and gas chromatography-mass spectrometry (GC-MS) analysis identified the main inhibitory active compounds as carvone in caraway and dill (seeds), 1,8-cineole in laurel and cardamom, and borneol in thyme. Both camphor and 1,8-cineole were detected in rosemary and sage, and the total activity evaluation showed that camphor was the major inhibitory compound in rosemary, although both compounds played equal roles in sage.
分别采用三明治法和培养皿装填法，评估了53种植物（香料和草本植物）的浸出液和挥发物对生菜（“大湖366”）幼苗生长的抑制活性。采用三明治法时，欧芹对生菜胚根生长的抑制作用最强（77%），其次是龙蒿（72%）。然而，在培养皿装填法中，葛缕子、莳萝（种子）、月桂、迷迭香和鼠尾草是抑制作用最强的物种（在所有距离的培养孔中，生菜胚根和下胚轴生长抑制率达100%）。小豆蔻和百里香也表现出较强的抑制活性（在所有41毫米和58毫米距离的培养孔中，胚根和下胚轴生长抑制率均达100%）。顶空采样和气相色谱 - 质谱联用（GC - MS）分析确定，主要的抑制活性化合物为葛缕子和莳萝（种子）中的香芹酮、月桂和小豆蔻中的1,8 - 桉叶素以及百里香中的冰片。迷迭香和鼠尾草中均检测到樟脑和1,8 - 桉叶素，总体活性评估表明，樟脑是迷迭香中的主要抑制化合物，不过这两种化合物在鼠尾草中的作用相当。
