Key Lab of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
College of Plant Science and Technology of Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2019 Oct 28;20(21):5355. doi: 10.3390/ijms20215355.
Salt stress inhibits the production of all crop species, including rapeseed ( L.), the second most widely planted oil crop species. Although melatonin was confirmed to alleviate salt stress in rapeseed seedlings recently, the mechanism governing the expression levels remains unknown. Therefore, the melatonin-induced transcriptome variation of salt-stressed seedlings was explored. In this study, the transcriptomes of leaves and roots under control (CK), salt (125 mM NaCl, ST) and melatonin (125 mM NaCl plus 50 µM melatonin, MS) treatments were evaluated by using next-generation sequencing techniques. After conducting comparisons of gene expression in the roots and leaves between MS and ST, the differentially expressed gene (DEG) pools were screened. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses highlighted the significant pathways, which were mainly related to plant hormone synthesis and signal transduction, lignin and fatty acid metabolism. The functional genes in the objective KEGG pathways were identified. Furthermore, members of several transcription factor (TF) families participated in the response process. Combined with the hormone (campesterol (CS), jasmonic acid (JA), and gibberellic acid 3 (GA3)) contents measured in the seedlings, it could be concluded that melatonin induced changes in the intrinsic hormone metabolic network, which promoted seedling growth. Thus, this study identified new candidate genes and pathways active during the interactions between melatonin and salt stress, which provide clues for disclosing melatonin's function in resistance to salt injury. Our results contribute to developing a practical method for sustainable agriculture on saline lands.
盐胁迫抑制所有作物物种的生长，包括油菜（L.），这是种植最广泛的第二大油料作物。尽管褪黑素最近被证实可以缓解油菜幼苗的盐胁迫，但控制其表达水平的机制尚不清楚。因此，本研究探索了褪黑素诱导盐胁迫下幼苗转录组的变化。在这项研究中，使用下一代测序技术评估了对照（CK）、盐（125 mM NaCl，ST）和褪黑素（125 mM NaCl 加 50 µM 褪黑素，MS）处理下叶片和根的转录组。在比较 MS 和 ST 处理下根和叶中的基因表达后，筛选出差异表达基因（DEG）库。京都基因与基因组百科全书（KEGG）富集分析突出了重要的途径，这些途径主要与植物激素合成和信号转导、木质素和脂肪酸代谢有关。确定了目标 KEGG 途径中的功能基因。此外，几个转录因子（TF）家族的成员参与了响应过程。结合幼苗中测量的激素（菜油甾醇（CS）、茉莉酸（JA）和赤霉素 3（GA3））含量，可以得出结论，褪黑素诱导了内在激素代谢网络的变化，从而促进了幼苗的生长。因此，本研究鉴定了褪黑素与盐胁迫相互作用过程中活跃的新候选基因和途径，为揭示褪黑素在耐盐损伤中的功能提供了线索。我们的研究结果有助于开发在盐渍地上可持续农业的实用方法。
