Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2023 Jul 8;24(14):11242. doi: 10.3390/ijms241411242.
Plant Cysteine Oxidase (PCO) is a plant O-sensing enzyme catalyzing the oxidation of cysteine to Cys-sulfinic acid at the N-termini of target proteins. To better understand the gene family, genes in and related species were analyzed. In this study, 20, 7 and 8 genes were identified in , and , respectively. According to phylogenetic analysis, the were divided into five groups: , , , and . Gene organization and motif distribution analysis suggested that the gene family was relatively conserved during evolution. According to the public expression data, genes were expressed in different tissues at different developmental stages. Moreover, qRT-PCR data showed that most of the members were expressed in leaves, roots, flowers and siliques, suggesting an important role in both vegetative and reproductive development. Expression of was induced by various abiotic stress, especially waterlogging stress, which was consistent with the result of cis-element analysis. In this study, the gene family of was analyzed for the first time, which contributes to a comprehensive understanding of the origin and evolution of genes in and the function of in abiotic stress responses.
植物半胱氨酸氧化酶 (PCO) 是一种植物 O 感应酶，可催化靶蛋白 N 末端的半胱氨酸氧化为半胱氨酸亚磺酸。为了更好地了解基因家族，分析了和相关物种中的基因。在这项研究中，分别在、和中鉴定出 20、7 和 8 个基因。根据系统发育分析，将基因分为五个组：、、、和。基因组织和基序分布分析表明，在进化过程中基因家族相对保守。根据公共表达数据，基因在不同发育阶段的不同组织中表达。此外，qRT-PCR 数据表明，大多数成员在叶片、根、花和角果中表达，这表明它们在营养和生殖发育中都具有重要作用。基因的表达受到各种非生物胁迫的诱导，特别是淹水胁迫，这与顺式元件分析的结果一致。本研究首次对的基因家族进行了分析，有助于全面了解和中基因的起源和进化以及基因在非生物胁迫反应中的功能。
