D'azeredo F, Meira-Filho M, Work T M
Econserv Diagnóstico e Consultoria, Pontal do Paraná - PR, 83255-000, Paraná, Brazil.
US Geological Survey, National Wildlife Health Center, Honolulu Field Station, PO Box 50167, Honolulu, HI 96850, USA.
Helminthologia. 2019 Sep 1;56(3):269-272. doi: 10.2478/helm-2019-0020. eCollection 2019 Sep.
The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae eggs/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm of tissue having better correlation.
本研究提出了一种用于量化动物组织中寄生虫卵的新方法。寄生虫的量化对于了解海龟体内旋尾虫感染的流行病学很重要，然而，在海龟组织中量化旋尾虫科虫卵时使用了不同的方法。量化组织中旋尾虫科负担的最具代表性的方法是计算每克组织中的虫卵数量，然而，这种方法非常费力。作为一种替代方法，我们建议在显微镜载玻片上量化旋尾虫科虫卵的数量/组织面积。我们将这种方法与每玻片虫卵数量进行了比较，这是海龟组织中虫卵负担的常用指标。两种方法与每克组织中的虫卵数量都有很好的相关性，其中每毫米组织中的虫卵数量相关性更好。
