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幼虫中该受体的分子特征

Molecular characterization of the receptor in larvae.

作者信息

Karamzadeh Tahereh, Alipour Hamzeh, Shahriari-Namadi Marziae, Raz Abbasali, Azizi Kourosh, Bagheri Masoumeh, Moemenbellah-Fard Mohammad D

机构信息

Research Center for Health Sciences, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran.

Department of Medical Entomology, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

AIMS Genet. 2019 Aug 8;6(3):46-54. doi: 10.3934/genet.2019.3.46. eCollection 2019.

Abstract

Larval therapy with is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. , one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The combines with a signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the mRNA in using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the netrin-1 . The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in . It lied at the interval of 26-131 aa. We identified the CDS of in . It could be applied to research activities implementing a new essential component design in wound healing.

摘要

使用幼虫疗法是伤口愈合中一种很有前景的策略。轴突导向分子在神经系统发育过程中发挥着至关重要的作用,并且在伤口愈合中也调节神经元修复的能力。幼虫分泌的蛋白质之一在细胞迁移和神经组织再生中发挥着有益作用。该蛋白质与一种信号结合并将信号从膜的一侧传递到另一侧,引发细胞活性的变化。在当前研究中,我们使用不同组的引物,包括外显子连接引物和特定区域引物,在[具体生物名称]中鉴定出了[蛋白质名称]mRNA的全长。对该[蛋白质名称]的编码序列(CDS)进行测序并鉴定,发现其包含633个碱基对的核酸,对其核苷酸序列进行BLAST分析显示与[具体生物名称]的netrin-1有96%的同一性。该蛋白质残基包含210个氨基酸(aa),编码一种分子量为24 kD的蛋白质。该基因缺乏信号肽。此外,UPA结构域在[蛋白质名称]中是保守的。它位于26 - 131 aa的区间。我们在[具体生物名称]中鉴定出了[蛋白质名称]的CDS。它可应用于在伤口愈合中实施新的关键成分设计的研究活动。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41e9/6803787/3783a89674c7/genetics-06-03-046-g001.jpg

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