inhibition of -carbonic anhydrase 3 with Mono- and dithiocarbamates and evaluation of their toxicity using zebrafish developing embryos.

We investigated a panel of 14 compounds belonging to the monothiocarbamate () and dithiocarbamate () series against the β-carbonic anhydrase 3 (-CA3) of (Mtb). We also evaluated all compounds for toxicity using 1-5-day post fertilisation zebrafish embryos. 11 out of the 14 investigated derivatives showed effective nanomolar or submicromolar inhibition against the -CA3 (Ks 2.4-812.0 nM), and among them four of the series ( and ) showed very significant inhibition potencies with Ks between 2.4 and 43 nM. Out of 14 compounds screened for toxicity and safety 9 compounds showed no adverse phenotypic effects on the developing zebrafish larvae at five days of exposure. The results of inhibition and the toxicological evaluation of our study suggest that 5 compounds are suitable for further preclinical characterisation in zebrafish model.