Department of Chemistry, Faculty of Science, Islamic Azad University Central Tehran Branch, Iran.
Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran.
Analyst. 2019 Dec 21;144(24):7336-7350. doi: 10.1039/c9an01654f. Epub 2019 Oct 30.
FeO nanoparticles with chitosan grafted onto poly(N-vinylcaprolactam) copolymers are synthesized and showed dual sensitivity to temperature and pH. The nanoparticles were characterized by different techniques and two aspects of their applications were evaluated using Imatinib mesylate drug as a model. The first studied application of these nanoparticles was the extraction and pre-concentration of Imatinib mesylate from biological samples. Parameters such as sorbent dosage and extraction and desorption time can affect Imatinib mesylate extraction and were studied by Box-Behnken design and response surface methodology, while the one-factor-at-a-time approach was used to assessed the sample pH and elution solvent type and amount. The determination and quantification were accomplished by ultra-performance liquid chromatography-mass spectrometry. The limit of quantification of the approach was 1.0 ng mL. Inter/intraday precision was obtained as 3.4-6.7% and 5.1-7.7%, respectively. Also, the modified nanoparticles were studied in basic in vitro drug delivery research which led to satisfactory results.
载有壳聚糖的 FeO 纳米粒子接枝到聚（N-乙烯基己内酰胺）共聚物上被合成出来，并表现出对温度和 pH 的双重敏感性。纳米粒子通过不同的技术进行了表征，并使用甲磺酸伊马替尼药物作为模型评估了它们的两个应用方面。这些纳米粒子的第一个研究应用是从生物样品中提取和预浓缩甲磺酸伊马替尼。吸附剂用量、萃取和洗脱时间等参数会影响甲磺酸伊马替尼的萃取，采用 Box-Behnken 设计和响应面法对这些参数进行了研究，而单因素法则用于评估样品 pH 值和洗脱溶剂的类型和用量。采用超高效液相色谱-质谱法进行了测定和定量。该方法的定量限为 1.0ngmL。日内/日间精密度分别为 3.4-6.7%和 5.1-7.7%。此外，还对基本的体外药物输送研究中的改性纳米粒子进行了研究，结果令人满意。
