Department of Chemistry, UGC Centre of Advanced Studies - II, Guru Nanak Dev University, Amritsar 143 005, India.
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, India.
J Mater Chem B. 2019 Dec 7;7(45):7218-7227. doi: 10.1039/c9tb02039j. Epub 2019 Oct 30.
The sustainable development of point-of-care testing (POCT) for spermine detection is important to check for food spoilage, early diagnosis of various malignancies and diminished anticonvulsant drug carbamazepine response in chronic epilepsy. Herein, the synthesis, characterization and spectroscopic properties of perylene diimide EA-PDI∩Cu complex based nanoparticles towards spermine were studied in detail. This EA-PDI∩Cu complex can be used for the ultrasensitive detection of spermine as low as 86.3 nM (UV-vis) and 90 pM (fluorescence) in aqueous medium, in urine and blood serum samples (recovery 99 ± 3) and in the solid state (0.1 μg L), and EA-PDI shows minimal cytotoxicity to cells and can easily enter into Human Osteosarcoma MG-63 cells for bio-imaging of Cu and spermine. This EA-PDI∩Cu complex can be established as a cost-effective method to develop a diagnostic kit for POCT of spermine in terms of a solution-based test kit for real time detection of spermine in vapor and solution form released from fermented food samples.
精氨酸检测的即时检验（POCT）的可持续发展对于检查食物腐败、各种恶性肿瘤的早期诊断以及慢性癫痫患者抗惊厥药物卡马西平反应降低非常重要。在此，详细研究了基于对苯二甲酰亚胺 EA-PDI∩Cu 配合物的纳米粒子对精氨酸的合成、表征和光谱特性。该 EA-PDI∩Cu 配合物可用于在水溶液中、尿液和血清样本中（回收率 99±3%）以及在固态（0.1μg L）中对精氨酸进行超灵敏检测，检测限低至 86.3 nM（紫外可见）和 90 pM（荧光），并且 EA-PDI 对细胞的细胞毒性极小，可轻易进入人骨肉瘤 MG-63 细胞，用于 Cu 和精氨酸的生物成像。该 EA-PDI∩Cu 配合物可作为一种具有成本效益的方法，用于开发 POCT 诊断试剂盒，用于实时检测发酵食品样品中释放的蒸汽和溶液形式的精氨酸。
