数据集：蛋白质组学过滤辅助样品制备的灵敏度和蛋白质消化过程。
Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation.
作者信息
Wiśniewski Jacek R, Zettl Katharina
机构信息
Max Planck Institute of Biochemistry, 82152, Martinsried, Germany.
出版信息
Data Brief. 2019 Sep 16;26:104530. doi: 10.1016/j.dib.2019.104530. eCollection 2019 Oct.
Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany "Filter Aided Sample Preparation - A Tutorial" (Wiśniewski, 2019).
使用来自HeLa细胞和小鼠大脑的SDS裂解物测试了FASP的灵敏度。使用QExactive HF-X仪器分析肽段。HeLa细胞的全细胞裂解物用FASP处理，采用单次或双次、连续或相继用LysC或胰蛋白酶消化。使用LTQ-Orbitrap质谱仪分析产生的肽段。这些数据集伴随“过滤辅助样品制备——教程”（维斯涅夫斯基，2019年）。
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