Wiśniewski Jacek R, Zettl Katharina
Max Planck Institute of Biochemistry, 82152, Martinsried, Germany.
Data Brief. 2019 Sep 16;26:104530. doi: 10.1016/j.dib.2019.104530. eCollection 2019 Oct.
Sensitivity of FASP was tested using SDS lysates from HeLa cells and mouse brain. Peptides were analyzed using a QExactive HF-X instrument. Whole cell lysates of Hela cells were processed with FASP using single or double, consecutive or successive, digestion with LysC or trypsin. The generated peptides were analyzed using a LTQ-Orbitrap mass spectrometer. These datasets accompany "Filter Aided Sample Preparation - A Tutorial" (Wiśniewski, 2019).
使用来自HeLa细胞和小鼠大脑的SDS裂解物测试了FASP的灵敏度。使用QExactive HF-X仪器分析肽段。HeLa细胞的全细胞裂解物用FASP处理，采用单次或双次、连续或相继用LysC或胰蛋白酶消化。使用LTQ-Orbitrap质谱仪分析产生的肽段。这些数据集伴随“过滤辅助样品制备——教程”（维斯涅夫斯基，2019年）。
