[工业环境下酶的催化作用及其适应性修饰：综述]
[Catalysis of enzymes under industrial environment and their adaptive modifications: a review].
作者信息
Wang Wenhao, Wen Pengfei, Xu Kongliang, Zheng Renchao, Zheng Yuguo
机构信息
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2019 Oct 25;35(10):1857-1869. doi: 10.13345/j.cjb.190258.
Enzymes have a wide range of applications and great industrial potential. However, large-scale applications of enzymes are restricted by the harsh industrial environment, such as high temperature, strong acid/alkali, high salt, organic solvents, and high substrate concentration. Adaptive modification (such as rational or semi-rational design, directed evolution and immobilization) is the most common strategy to improve the catalysis of enzymes under industrial conditions. Here, we review the catalysis of enzymes in the industrial environment and various methods adopted for the adaptive modifications in recent years, to provide reference for the adaptive modifications of enzymes.
酶具有广泛的应用和巨大的工业潜力。然而，酶的大规模应用受到恶劣工业环境的限制，如高温、强酸/强碱、高盐、有机溶剂和高底物浓度。适应性修饰（如理性或半理性设计、定向进化和固定化）是改善酶在工业条件下催化作用的最常用策略。在此，我们综述了酶在工业环境中的催化作用以及近年来用于适应性修饰的各种方法，为酶的适应性修饰提供参考。
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