Coimbra Diogo P, Penedo Diego M, Silva Monique O M, Abreu Ana P M, Silva Cláudia B, Verona Carlos E, Heliodoro Gabriela C, Massard Carlos L, Nogueira Denise M
Programa de Pós-graduação em Biologia Animal (PPGBA), Instituto de Ciências Biológicas e da Saúde (ICBS), Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ 23897-000, Brazil.
Programa de Pós-graduação em Biologia Animal (PPGBA), Instituto de Ciências Biológicas e da Saúde (ICBS), Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ 23897-000, Brazil.
Parasitol Int. 2020 Apr;75:101999. doi: 10.1016/j.parint.2019.101999. Epub 2019 Oct 24.
Callithrix jacchus and C. penicillata marmosets are invasive to the state of Rio de Janeiro, Brazil, threatening the native and vulnerable C. aurita. Both invasive species can be hosts of Trypanosoma cruzi, T. minasense, T. rangeli and T. devei. We aim to investigate the occurrence of trypanosomatids in Callithrix sp. from Jardim Botânico do Rio de Janeiro, located in a central and populous area of the city. Fifteen marmosets were captured. Blood samples were collected for light microscopy and molecular genetics analysis. Parasites morphometric values were evaluated for species identification. DNA was extracted from blood samples by phenol-chloroform method, for partial amplification of the 18S rRNA gene. PCR products were sequenced and aligned using BLAST®. A maximum likelihood phylogenetic tree was constructed to analyze the proximity between the observed sequences. By light microscopy, trypomastigotes were detected in five of the fifteen marmosets. Morphometric measurements and size polymorphism corresponded to those previously described for T. minasense. The DNA sequences of approximately 600 base pairs of the 18S rRNA gene were obtained for three samples with 99% identity with T. minasense sequence, forming a cluster in the phylogenetic tree and corroborating morphometric analysis. Trypanosoma minasense is a highly specific parasite to non-human primates considered as non-pathogenic. There is no evidence of infection in humans and these parasite findings from invasive marmosets do not support additional risks for the native species.
普通狨猴和白领伶猴对巴西里约热内卢州具有入侵性，威胁到了当地濒危的金毛狨猴。这两种入侵物种都可能是克氏锥虫、米纳斯锥虫、兰氏锥虫和德维锥虫的宿主。我们旨在调查位于里约热内卢市中心人口密集地区的里约热内卢植物园内普通狨猴属物种中锥虫的存在情况。捕获了15只狨猴。采集血样用于光学显微镜检查和分子遗传学分析。评估寄生虫形态测量值以进行物种鉴定。采用酚-氯仿法从血样中提取DNA，用于18S rRNA基因的部分扩增。对PCR产物进行测序，并使用BLAST®进行比对。构建最大似然系统发育树以分析观察到的序列之间的亲缘关系。通过光学显微镜检查，在15只狨猴中有5只检测到了锥鞭毛体。形态测量和大小多态性与先前描述的米纳斯锥虫一致。从三个样本中获得了18S rRNA基因约600个碱基对的DNA序列，与米纳斯锥虫序列的同一性为99%，在系统发育树中形成一个聚类，证实了形态测量分析。米纳斯锥虫是一种高度特异性的非人灵长类寄生虫，被认为是非致病性的。没有人类感染的证据，并且从入侵狨猴中发现的这些寄生虫不支持对本地物种有额外风险。
