Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, 999 Phutthamonthon 4 Road, Salaya, Nakhon Pathom, 73170, Thailand.
Department of Microbiology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Patumwan, Bangkok, 10330, Thailand.
J Pharm Biomed Anal. 2020 Jan 30;178:112925. doi: 10.1016/j.jpba.2019.112925. Epub 2019 Oct 19.
The measurement of cortisol (stress hormone) is important for diagnosis and monitoring of stress-related diseases. A paper-based immunosensor with a competitive assay was developed for quick and easy detection of cortisol levels in serum. The paper-based sensor was fabricated using a simple and cheap wax printing method. Cortisol conjugated-BSA was immobilized on the paper's surface in the detection zone for the competitive immunoassay. Anti-cortisol mAb-conjugated gold nanoparticles, as signal indicator, were used to detect cortisol in the sample. A 2-step procedure included applying the sample and washing for cortisol determination. The results showed that the device had the ability to differentiate cortisol levels into three ranges: < 25 μg/dL, 25-50 μg/dL and > 50 μg/dL by visual detection. The limit of detection determined by an image processing program was 21.5 μg/dL. This assay was successfully developed to detect cortisol in serum, and showed good recovery and precision. Our results correlated well with those obtained using an electrochemiluminescence method. This paper-based immunosensor provided a rapid and simple screening test for serum cortisol detection.
皮质醇（应激激素）的测量对于应激相关疾病的诊断和监测很重要。本研究开发了一种基于纸的免疫传感器，采用竞争分析法，可快速、简便地检测血清中的皮质醇水平。纸基传感器采用简单且廉价的蜡印方法制备。在检测区，将皮质醇结合-BSA 固定在纸的表面，用于竞争性免疫分析。抗皮质醇单克隆抗体偶联的金纳米粒子作为信号指示剂，用于检测样品中的皮质醇。两步法包括加样和洗涤过程，用于皮质醇的测定。结果表明，该装置通过目测具有区分皮质醇水平为三个范围的能力：<25μg/dL、25-50μg/dL 和 >50μg/dL。图像处理程序确定的检测限为 21.5μg/dL。该方法成功用于检测血清中的皮质醇，显示出良好的回收率和精密度。我们的结果与电化学发光法的结果相关性良好。这种基于纸的免疫传感器为血清皮质醇检测提供了一种快速简便的筛选试验。
