Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan, 98135-674, Iran.
Department of Chemistry, Faculty of Sciences, University of Sistan and Baluchestan, Zahedan, 98135-674, Iran; Smartphone Analytical Sensors Research Centre, University of Sistan and Baluchestan, Zahedan, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 15;227:117672. doi: 10.1016/j.saa.2019.117672. Epub 2019 Oct 19.
In this paper, a low cost and portable smartphone-based spectrophotometer with the purpose of measuring trace levels of two important anions, chlorine and nitrite ions in water samples, is introduced. This home-made spectrophotometer is made of Plexiglas, equipped with two LEDs as a light source, and a piece of DVD is acted as light dispersing element. Battery of smartphone was used as its power supply and spectral analysis was performed by a free software downloadable from Google Playstore. By using this lightweight spectrophotometer, various environmental samples were analyzed for their NO and Cl content in field. Good detection limits of 5.00 × 10 mg L and 8.60 × 10 mg L were obtained for chlorine and nitrite, respectively. The linear range for chlorine was 1.00-4.00 mg L and this range for nitrite was 0.05-1.20 mg L. Reproducibility as relative standard deviation for both chlorine and nitrite was better than 8.75%. In order to investigate validity of data, results were compared to standard methods of measuring chlorine and nitrite, using both spectrophotometry and commercial kits which showed no difference between results obtained. This very simple to use and inexpensive device can be used many times, so can be considered as a low-cost alternative to the detection device of commercial kits.
本文介绍了一种低成本、便携式的智能手机分光光度计，用于测量水样中两种重要阴离子（氯和亚硝酸盐离子）的痕量水平。这种自制的分光光度计由有机玻璃制成，配备了两个作为光源的 LED，以及一块 DVD 作为分光元件。智能手机的电池用作其电源，光谱分析由可从 Google Playstore 下载的免费软件完成。使用这种轻便的分光光度计，在现场对各种环境样品进行了 NO 和 Cl 含量的分析。氯的检测限为 5.00×10mg/L，亚硝酸盐的检测限为 8.60×10mg/L。氯的线性范围为 1.00-4.00mg/L，亚硝酸盐的线性范围为 0.05-1.20mg/L。氯和亚硝酸盐的相对标准偏差（RSD）均优于 8.75%。为了验证数据的有效性，使用分光光度法和商业试剂盒对氯和亚硝酸盐的标准方法进行了比较，结果没有差异。这种非常简单易用、价格低廉的设备可以多次使用，因此可以作为商业试剂盒检测设备的低成本替代品。
