CLARITY: Centre for Sensor Web Technologies, National Centre for Sensor Research, Dublin City University, Dublin, Ireland.
Talanta. 2013 Nov 15;116:997-1004. doi: 10.1016/j.talanta.2013.07.058. Epub 2013 Aug 20.
A wireless, portable, fully-integrated microfluidic analytical platform has been developed and applied to the monitoring and determination of nitrite anions in water, using the Griess method. The colour intensity of the Griess reagent nitrite complex is detected using a low cost Paired Emitter Detector Diode, while on-chip fluid manipulation is performed using a biomimetic photoresponsive ionogel microvalve, controlled by a white light LED. The microfluidic analytical platform exhibited very low limits of detection (34.0±0.1 μg L(-1) of NO2(-)). Results obtained with split freshwater samples showed good agreement between the microfluidic chip platform and a conventional UV-vis spectrophotometer (R(2)=0.98, RSD=1.93% and R(2)=0.99, RSD=1.57%, respectively). The small size, low weight, and low cost of the proposed microfluidic platform coupled with integrated wireless communications capabilities make it ideal for in situ environmental monitoring. The prototype device allows instrument operational parameters to be controlled and analytical data to be downloaded from remote locations. To our knowledge, this is the first demonstration of a fully functional microfluidic platform with integrated photo-based valving and photo-detection.
已开发出一种无线、便携、完全集成的微流控分析平台，并将其应用于使用格里斯（Griess）法监测和测定水中的亚硝酸盐阴离子。使用低成本的成对发射器检测器二极管检测格里斯试剂亚硝酸盐络合物的颜色强度，而芯片上的流体处理则使用仿生光响应离子凝胶微阀进行，由白光 LED 控制。微流控分析平台表现出非常低的检测极限（34.0±0.1 μg L(-1)的 NO2(-)）。用分离的淡水样品得到的结果表明，微流控芯片平台与传统的紫外-可见分光光度计之间具有良好的一致性（R(2)=0.98，RSD=1.93%和 R(2)=0.99，RSD=1.57%，分别）。所提出的微流控平台具有体积小、重量轻、成本低的特点，并具有集成的无线通信功能，非常适合现场环境监测。该原型设备允许控制仪器操作参数，并从远程位置下载分析数据。据我们所知，这是首次展示具有集成基于光的阀和光检测功能的全功能微流控平台。
