Jiangxi Key Laboratory of Natural Product and Functional Food, School of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China.
School of Foreign Languages, Jiangxi Agricultural University, Nanchang, 330045, China.
Food Funct. 2019 Nov 1;10(11):7489-7497. doi: 10.1039/c9fo00502a. Epub 2019 Oct 31.
To investigate the mechanism of the combined effects of chlorogenic acid (CGA) and caffeine on lipid metabolism in high-fat diet-induced obese mice, eighty female ICR mice were randomly divided into eight groups and fed with a high-fat diet with/without CGA and/or caffeine for 14 weeks. The combination of CGA and caffeine effectively decreased body weight gain, intraperitoneal adipose tissue weight, serum LDL-c, FFA, TC, TG, leptin, IL-6 concentrations, and hepatic TG and TC levels and increased the serum adiponectin level. The CGA and caffeine combination also promoted the phosphorylation of AMPKα, inhibited the expressions of transcriptional regulators (SREBP-1c and LXRα), and decreased the expressions of FAS and HMGR. Besides, the expressions of ACO, ATGL and HSL were increased by the CGA and caffeine combinations. The results indicated that the combination of CGA and caffeine had anti-obesity effects and regulated lipid metabolism in high-fat diet-induced obese mice via the AMPKα-LXRα/SREBP-1c signaling pathway. Thus, chronic CGA and caffeine intakes may be potent for preventing obesity.
为了研究绿原酸（CGA）和咖啡因联合作用对高脂饮食诱导肥胖小鼠脂代谢的机制，将 80 只雌性 ICR 小鼠随机分为 8 组，分别给予高脂饮食加/不加 CGA 和/或咖啡因喂养 14 周。CGA 和咖啡因联合使用可有效降低体重增加、腹腔脂肪组织重量、血清 LDL-c、FFA、TC、TG、瘦素、IL-6 浓度以及肝 TG 和 TC 水平，并升高血清脂联素水平。CGA 和咖啡因联合使用还可促进 AMPKα 的磷酸化，抑制转录调节因子（SREBP-1c 和 LXRα）的表达，降低 FAS 和 HMGR 的表达。此外，CGA 和咖啡因联合使用可增加 ACO、ATGL 和 HSL 的表达。结果表明，CGA 和咖啡因联合使用具有抗肥胖作用，并通过 AMPKα-LXRα/SREBP-1c 信号通路调节高脂饮食诱导肥胖小鼠的脂代谢。因此，长期摄入 CGA 和咖啡因可能具有预防肥胖的潜力。
