State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
Toxins (Basel). 2019 Oct 29;11(11):628. doi: 10.3390/toxins11110628.
causes head blight (FHB), a devastating disease of wheat. Salicylic acid (SA) is involved in the resistance of wheat to . Cell wall mannoprotein (CWM) is known to trigger defense responses in plants, but its role in the pathogenicity of remains unclear. Here, we characterized (), encoding a CWM in . was highly expressed in wheat spikes by 24 h after initial inoculation and was upregulated by SA. Disruption of (Δ) reduced mannose and protein accumulation in the fungal cell wall, especially under SA treatment, and resulted in defective fungal cell walls, leading to increased fungal sensitivity to SA. The positive role of in mannose and protein accumulation was confirmed by its expression in . Compared with wild type (WT), Δ exhibited reduced pathogenicity toward wheat, but it produced the same amount of deoxynivalenol both in culture and in spikes. Complementation of Δ with restored the WT phenotype. Localization analyses revealed that FgCWM1 was distributed on the cell wall, consistent with its structural role. Thus, encodes a CWM protein that plays an important role in the cell wall integrity and pathogenicity of .
引起小麦赤霉病（FHB），这是一种毁灭性的小麦疾病。水杨酸（SA）参与了小麦对 的抗性。细胞壁甘露糖蛋白（CWM）已知会在植物中引发防御反应，但它在 的致病性中的作用尚不清楚。在这里，我们对 （）进行了表征，该基因编码了小麦中的一种 CWM。 在初始接种后 24 小时， 在小麦穗中高度表达，并被 SA 上调。 （Δ）的破坏减少了真菌细胞壁中的甘露糖和蛋白质积累，特别是在 SA 处理下，导致真菌细胞壁缺陷，使真菌对 SA 的敏感性增加。通过在 中表达 进一步证实了其在甘露糖和蛋白质积累中的积极作用。与野生型（WT）相比，Δ对小麦的致病性降低，但它在培养和穗中产生的脱氧雪腐镰刀菌烯醇量相同。用 互补 Δ 恢复了 WT 表型。定位分析表明，FgCWM1 分布在细胞壁上，与其结构作用一致。因此， 编码一种 CWM 蛋白，在 的细胞壁完整性和致病性中发挥重要作用。
