Departments of Neurology, Hanyang University Guri Hospital, 11923, Guri, Korea.
Departments of Neurosurgery, Hanyang University Guri Hospital, 11923, Guri, Korea.
Sci Rep. 2019 Oct 31;9(1):15717. doi: 10.1038/s41598-019-52083-y.
Amlodipine, a L-type calcium channel blocker, has been reported to have a neuroprotective effect in brain ischemia. Mitochondrial calcium overload leads to apoptosis of cells in neurologic diseases. We evaluated the neuroprotective effects of amlodipine camsylate (AC) on neural stem cells (NSCs) injured by oxygen glucose deprivation (OGD) with a focus on mitochondrial structure and function. NSCs were isolated from rodent embryonic brains. Effects of AC on cell viability, proliferation, level of free radicals, and expression of intracellular signaling proteins were assessed in OGD-injured NSCs. We also investigated the effect of AC on mitochondrial structure in NSCs under OGD by transmission electron microscopy. AC increased the viability and proliferation of NSCs. This beneficial effect of AC was achieved by strong protection of mitochondria. AC markedly enhanced the expression of mitochondrial biogenesis-related proteins and mitochondrial anti-apoptosis proteins. Together, our results indicate that AC protects OGD-injured NSCs by protecting mitochondrial structure and function. The results of the present study provide insight into the mechanisms underlying the protective effects of AC on NSCs.
氨氯地平是一种 L 型钙通道阻滞剂，据报道在脑缺血中有神经保护作用。线粒体钙超载导致神经疾病中的细胞凋亡。我们评估了氨氯地平 camsylate（AC）对氧葡萄糖剥夺（OGD）损伤的神经干细胞（NSC）的神经保护作用，重点关注线粒体的结构和功能。NSC 从啮齿动物胚胎脑中分离出来。在 OGD 损伤的 NSC 中评估 AC 对细胞活力、增殖、自由基水平和细胞内信号蛋白表达的影响。我们还通过透射电子显微镜研究了 AC 在 OGD 下对 NSC 中线粒体结构的影响。AC 增加了 NSC 的活力和增殖。AC 通过对线粒体的强烈保护来实现这种有益的效果。AC 显著增强了与线粒体生物发生相关的蛋白质和线粒体抗凋亡蛋白的表达。总之，我们的结果表明 AC 通过保护线粒体的结构和功能来保护 OGD 损伤的 NSC。本研究的结果为 AC 对 NSC 的保护作用的机制提供了深入的了解。
