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L. cv. Takanari的全基因组测序数据。

Whole genome sequencing data of L. cv. Takanari.

作者信息

Kajiya-Kanegae Hiromi, Yabe Shiori, Yoshida Hiroe, Ebana Kaworu, Yamasaki Masanori, Nakagawa Hiroshi, Iwata Hiroyoshi

机构信息

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Science, The University of Tokyo, Japan.

Institute of Crop Science, NARO, Japan.

出版信息

Data Brief. 2019 Sep 20;27:104546. doi: 10.1016/j.dib.2019.104546. eCollection 2019 Dec.

DOI:10.1016/j.dib.2019.104546
PMID:31673576
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6817641/
Abstract

L. 'Takanari' is one of the most productive cultivars [1,2]. Reciprocal chromosome segment substitution lines (CSSLs) derived from a cross between 'Koshihikari' and 'Takanari' are useful tools for the detection and precise mapping of target quantitative trait loci (QTL) in 'Takanari'. Although the available Os-Nipponbare-Reference-IRGSP-1.0 reference genome is available and useful for evaluating genetic diversity among cultivars, it is not always useful for evaluating genetic diversity harbored by cultivars such as 'Takanari'. To reveal sequence variants in 'Takanari' and to exploit these variants in rice breeding programs, the whole genome of 'Takanari' was sequenced using a combination of Illumina HiSeq X Ten (20,983,495 reads and %GC 43) and PacBio (2,847,220 high-quality subreads). NGS data obtained have been deposited in the DNA Data Bank of Japan (DDBJ) under accession number DRA007557.

摘要

“丰锦”是产量最高的品种之一[1,2]。由“越光”和“丰锦”杂交产生的相互染色体片段代换系(CSSLs)是检测和精确定位“丰锦”中目标数量性状基因座(QTL)的有用工具。尽管现有的日本晴参考基因组Os-Nipponbare-Reference-IRGSP-1.0可用于评估品种间的遗传多样性,但它并不总是有助于评估“丰锦”等品种所具有的遗传多样性。为了揭示“丰锦”中的序列变异并在水稻育种计划中利用这些变异,我们结合Illumina HiSeq X Ten(20,983,495条 reads,%GC 43)和PacBio(2,847,220条高质量子reads)对“丰锦”的全基因组进行了测序。获得的NGS数据已存入日本DNA数据库(DDBJ),登录号为DRA007557。

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本文引用的文献

1
The Third Revolution in Sequencing Technology.测序技术的第三次革命。
Trends Genet. 2018 Sep;34(9):666-681. doi: 10.1016/j.tig.2018.05.008. Epub 2018 Jun 22.
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PacBio Sequencing and Its Applications.PacBio测序技术及其应用。
Genomics Proteomics Bioinformatics. 2015 Oct;13(5):278-89. doi: 10.1016/j.gpb.2015.08.002. Epub 2015 Nov 2.
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Genetic mechanisms underlying yield potential in the rice high-yielding cultivar Takanari, based on reciprocal chromosome segment substitution lines.基于相互染色体片段代换系解析水稻高产栽培品种“日本晴”产量潜力的遗传机制
BMC Plant Biol. 2014 Nov 18;14:295. doi: 10.1186/s12870-014-0295-2.
4
Improvement of the Oryza sativa Nipponbare reference genome using next generation sequence and optical map data.利用下一代测序和光学图谱数据提高水稻日本晴参考基因组质量。
Rice (N Y). 2013 Feb 6;6(1):4. doi: 10.1186/1939-8433-6-4.
5
Hydraulic conductance as well as nitrogen accumulation plays a role in the higher rate of leaf photosynthesis of the most productive variety of rice in Japan.水力传导率以及氮积累在日本最具生产力的水稻品种中具有更高的叶片光合作用速率方面发挥了作用。
J Exp Bot. 2011 Jul;62(11):4067-77. doi: 10.1093/jxb/err126. Epub 2011 Apr 28.