Knighton Laura E, Wolfgeher Donald, Reitzel Adam M, Truman Andrew W
Department of Biological Sciences, The University of North Carolina at Charlotte, Charlotte, USA.
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, USA.
Data Brief. 2019 Sep 28;27:104580. doi: 10.1016/j.dib.2019.104580. eCollection 2019 Dec.
is an estuarine sea anemone that has emerged as a model species to characterize molecular responses to physiological stressors due to its exposure to diverse, extreme abiotic conditions. In marine cnidarians, Hsp70 proteins can be effective biomarkers to determine mechanisms of physiological acclimation and evolutionary adaptations to environmental stress: a pressing issue as concerns about climate change grow. Here we show the results of affinity purification mass spectrometry of three Hsp70 isoforms, NvHsp70A, B and D when expressed in untreated and heat shocked yeast cells lacking their native Hsp70s. We identified a total of 1031 interactors for the three NvHsp70 isoforms, 549 or which were shared. NvHsp70 isoform interactions altered substantially under heat stress with 17% of NvHsp70A, 51% of NvHsp70B and 20% of NvHsp70D interactions increasing after exposure to 39 °C for 2 hours. For further interpretation of the data presented in this article, please see the research article "Dynamic remodeling of the interactomes of Hsp70 isoforms under heat shock.
是一种河口海葵，由于其暴露于多样、极端的非生物条件下，已成为表征对生理应激源分子反应的模式物种。在海洋刺胞动物中，热休克蛋白70（Hsp70）可作为有效的生物标志物，用于确定生理适应机制以及对环境压力的进化适应：随着对气候变化的担忧加剧，这是一个紧迫的问题。在这里，我们展示了三种Hsp70异构体NvHsp70A、B和D在缺乏天然Hsp70的未处理和热激酵母细胞中表达时的亲和纯化质谱分析结果。我们总共鉴定出三种NvHsp70异构体的1031个相互作用蛋白，其中549个是共享的。在热应激下，NvHsp70异构体的相互作用发生了显著变化，在暴露于39°C 2小时后，17%的NvHsp70A、51%的NvHsp70B和20%的NvHsp70D相互作用增加。有关本文数据的进一步解释，请参阅研究文章《热休克下Hsp70异构体相互作用组的动态重塑》。
