Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 52900, Israel.
Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.
Int J Mol Sci. 2021 Jul 12;22(14):7454. doi: 10.3390/ijms22147454.
Organisms' survival is associated with the ability to respond to natural or anthropogenic environmental stressors. Frequently, these responses involve changes in gene regulation and expression, consequently altering physiology, development, or behavior. Here, we present modifications in response to heat exposure that mimics extreme summertime field conditions of lab-cultured and field-conditioned . Using ATAC-seq and RNA-seq data, we found that field-conditioned animals had a more concentrated reaction to short-term thermal stress, expressed as enrichment of the DNA repair mechanism pathway. By contrast, lab animals had a more diffuse reaction that involved a larger number of differentially expressed genes and enriched pathways, including amino acid metabolism. Our results demonstrate that pre-conditioning affects the ability to respond efficiently to heat exposure in terms of both chromatin accessibility and gene expression and reinforces the importance of experimentally addressing ecological questions in the field.
生物的生存与其应对自然或人为环境胁迫的能力有关。通常，这些反应涉及基因调控和表达的变化，从而改变生理、发育或行为。在这里，我们展示了对热暴露的反应的改变，这种改变模拟了实验室培养和野外驯化的. 使用 ATAC-seq 和 RNA-seq 数据，我们发现野外驯化的动物对短期热应激有更集中的反应，表现为 DNA 修复机制途径的富集。相比之下，实验室动物的反应更为分散，涉及到更多差异表达基因和富集途径，包括氨基酸代谢。我们的研究结果表明，预驯化会影响生物在染色质可及性和基因表达方面对热暴露的有效反应能力，并强调了在野外实验解决生态问题的重要性。
