Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment & Technology, State Key Laboratory of Food Science and Technology, School of Food Science and Technology , Jiangnan University , Nanjing 210037 , Jiangsu , China.
Department of Food Science and Engineering, College of Light Industry and Food Engineering , Nanjing Forestry University , Nanjing 210037 , Jiangsu , China.
J Agric Food Chem. 2019 Dec 4;67(48):13299-13306. doi: 10.1021/acs.jafc.9b05829. Epub 2019 Nov 14.
l-Arabinose is a monosaccharide extracted from plants or fibers, which is known to have a variety of functional properties. In this study, we aim to investigate whether l-arabinose could inhibit colitis by modulating gut microbiota. l-Arabinose was administered in mice daily in a dextran sodium sulfate (DSS)-induced colitis model. The histological analysis, disease index, and the expression of inflammatory genes were measured. 16S-rRNA sequence analysis was performed to investigate gut microbiota. Intriguingly, we found that l-arabinose could repress DSS-induced colitis and inhibit p38-/p65-dependent inflammation activation. Besides that, our data revealed that l-arabinose-modulated DSS-induced gut microbiota were disturbed. Additionally, the perturbed gut microbiota was responsible for the suppressive effects of l-arabinose on DSS-induced colitis treated with antibiotics. Lastly, Caco-2 cells were used to confirm the protective effects of l-arabinose in colitis or inflammatory bowel disease. As expected, the protein expression levels in Caco-2 cells of pro-inflammatory genes, which were treated with l-arabinose and incubated with or without tumor necrosis factor alpha. Our work suggested that l-arabinose exerts anti-inflammation effects in DSS-induced colitis. These beneficial effects have correlations with the composition, diversity, and abundance of the gut microbiota regulated by l-arabinose. l-Arabinose could be a remarkable candidate as a functional food or novel therapeutic strategy for intestinal health.
阿拉伯糖是一种从植物或纤维中提取的单糖，具有多种功能特性。在这项研究中，我们旨在研究阿拉伯糖是否可以通过调节肠道微生物群来抑制结肠炎。在葡聚糖硫酸钠（DSS）诱导的结肠炎模型中，每天给小鼠施用阿拉伯糖。测量组织学分析、疾病指数和炎症基因的表达。进行 16S-rRNA 序列分析以研究肠道微生物群。有趣的是，我们发现阿拉伯糖可以抑制 DSS 诱导的结肠炎并抑制 p38-/p65 依赖性炎症激活。此外，我们的数据显示，阿拉伯糖调节的 DSS 诱导的肠道微生物群受到干扰。此外，受干扰的肠道微生物群是阿拉伯糖对用抗生素处理的 DSS 诱导的结肠炎的抑制作用的原因。最后，使用 Caco-2 细胞来确认阿拉伯糖在结肠炎或炎症性肠病中的保护作用。正如预期的那样，用阿拉伯糖处理并用或不用肿瘤坏死因子-α孵育的 Caco-2 细胞中的促炎基因的蛋白表达水平。我们的工作表明，阿拉伯糖在 DSS 诱导的结肠炎中发挥抗炎作用。这些有益作用与阿拉伯糖调节的肠道微生物群的组成、多样性和丰度有关。阿拉伯糖可能是一种有前途的功能性食品或新型治疗肠道健康的策略。
