Division of Dermatology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California.
Department of Psychiatry, Semel Institute for Neuroscience and Behavior, University of California, Los Angeles, Los Angeles, California.
J Invest Dermatol. 2020 Apr;140(4):764-773.e4. doi: 10.1016/j.jid.2019.07.726. Epub 2019 Oct 31.
The epidermis and its appendage, the hair follicle, represent an elegant developmental system in which cells are replenished with regularity because of controlled proliferation, lineage specification, and terminal differentiation. Although transcriptome data exists for human epidermal and dermal cells, the hair follicle remains poorly characterized. Through single-cell resolution profiling of the epidermis and anagen hair follicle, we characterized the anatomical, transcriptional, functional, and pathological profiles of distinct epidermal, hair follicle, and hair follicle-associated cell subpopulations including melanocytes, endothelial cells, and immune cells. We additionally traced the differentiation trajectory of interfollicular and matrix cell progenitors and explored the association of specific cell subpopulations to known molecular signatures of common skin conditions. These data simultaneously corroborate prior murine and human studies while offering new insights into epidermal and hair follicle differentiation and pathogenesis.
表皮及其附属物毛囊是一个优雅的发育系统，其中细胞通过有规律的控制增殖、谱系特化和终末分化得到补充。尽管人类表皮和真皮细胞的转录组数据已经存在，但毛囊仍然知之甚少。通过对表皮和生长期毛囊进行单细胞分辨率分析，我们描述了不同表皮、毛囊和毛囊相关细胞亚群（包括黑素细胞、内皮细胞和免疫细胞）的解剖学、转录组、功能和病理学特征。我们还追踪了毛囊间充质和基质细胞前体的分化轨迹，并探讨了特定细胞亚群与常见皮肤疾病的已知分子特征之间的关联。这些数据既与之前的鼠类和人类研究相吻合，又为表皮和毛囊的分化和发病机制提供了新的见解。
