Li Ruikang, Li Jiashan, Liu Shuai, Guo Xinya, Lu Jianyu, Wang Tao, Chen Junjie, Zheng Yue, Yuan Yue, Du Jiaxin, Zhu Bolin, Wei Xiaoyu, Guo Pengcheng, Liu Longqi, Xu Xun, Dai Xi, Huang Runzhi, Liu Xin, Hu Xiaoyan, Wang Shiwei, Ji Shizhao
Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology of Shaanxi Province, The College of Life Sciences, Northwest University, Xi'an, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Front Cell Dev Biol. 2025 Jan 7;12:1519926. doi: 10.3389/fcell.2024.1519926. eCollection 2024.
Burn injuries often leave behind a "stasis zone", a region of tissue critically important for determining both the severity of the injury and the potential for recovery. To understand the intricate cellular and epigenetic changes occurring within this critical zone, we utilized single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) to profile over 31,500 cells from both healthy rat skin and the stasis zone at nine different time points after a burn injury. This comprehensive approach revealed 26 distinct cell types and the dynamic shifts in the proportions of these cell types over time. We observed distinct gene activation patterns in different cell types at various stages post-burn, highlighting key players in immune activation, tissue regeneration, and blood vessel repair. Importantly, our analysis uncovered the regulatory networks governing these genes, offering valuable insights into the intricate mechanisms orchestrating burn wound healing. This comprehensive cellular and molecular atlas of the stasis zone provides a powerful resource for developing targeted therapies aimed at improving burn injury recovery and minimizing long-term consequences.
烧伤常常会留下一个“停滞区”，该组织区域对于确定损伤的严重程度和恢复潜力至关重要。为了了解这个关键区域内发生的复杂细胞和表观遗传变化，我们利用单细胞转座酶可及染色质测序（scATAC-seq）对烧伤后九个不同时间点的健康大鼠皮肤和停滞区的31500多个细胞进行了分析。这种全面的方法揭示了26种不同的细胞类型以及这些细胞类型的比例随时间的动态变化。我们观察到烧伤后不同阶段不同细胞类型中不同的基因激活模式，突出了免疫激活、组织再生和血管修复中的关键参与者。重要的是，我们的分析揭示了调控这些基因的网络，为协调烧伤创面愈合的复杂机制提供了有价值的见解。这个停滞区的全面细胞和分子图谱为开发旨在改善烧伤恢复和最小化长期后果的靶向治疗提供了强大的资源。
