YoungLai E V, Wilkinson M, Hibbert P, McMahon A, Jarrell J F
Department of Obstetrics and Gynecology, McMaster University, Hamilton, Ont., Canada.
Can J Physiol Pharmacol. 1988 Apr;66(4):337-41. doi: 10.1139/y88-058.
The binding of 125I-labelled human chorionic gonadotropin (HCG) was studied using thick slices (300 micron) of rabbit ovarian tissue. Binding was saturable, reversible, stereospecific, and of high affinity. The amount of binding was proportional to the number of slices used and could be destroyed by boiling. Ovarian slices from eight individual rabbits were found to have two binding sites for 125I-labelled HCG with KD values of 272 +/- 64 and 1263 +/- 274 pM and Bmax values of 25.7 +/- 5.3 and 94.1 +/- 18.8 fmol/mg protein, respectively. In a comparative study the KD and Bmax values were 351 +/- 151 pM and 25.3 +/- 11.1 fmol/mg protein with slices from one ovary and 134 +/- 24 pM and 109 +/- 32 fmol/mg protein with membranes from the contralateral ovary. These data suggest that the binding of HCG can be determined in live tissue.
使用兔卵巢组织的厚切片（300微米）研究了125I标记的人绒毛膜促性腺激素（HCG）的结合情况。结合具有饱和性、可逆性、立体特异性和高亲和力。结合量与所用切片数量成正比，且可通过煮沸破坏。发现来自八只不同兔子的卵巢切片对125I标记的HCG有两个结合位点，其解离常数（KD）值分别为272±64和1263±274皮摩尔，最大结合容量（Bmax）值分别为25.7±5.3和94.1±18.8飞摩尔/毫克蛋白质。在一项对比研究中，来自一个卵巢的切片的KD和Bmax值分别为351±151皮摩尔和25.3±11.1飞摩尔/毫克蛋白质，而来自对侧卵巢的膜的KD和Bmax值分别为134±24皮摩尔和109±32飞摩尔/毫克蛋白质。这些数据表明，可以在活组织中测定HCG的结合情况。
