Bortolussi M, Selmin O, Colombatti A
J Immunoassay. 1987;8(2-3):219-35. doi: 10.1080/15321818708057023.
A solid-phase radioligand-receptor assay (RRA) to measure the binding of 125I-labelled human chorionic gonadotropin (125I-hCG) to target cell membranes has been developed. The binding of 125I-hCG to membranes immobilized on the wells of microtitration plates reached a maximum at about 3 hours at 37 degrees C, was saturable, displayed a high affinity (Ka = 2.4 X 10(9) M-1) and was specifically inhibited by unlabelled hCG. In comparison with RRAs carried out with membranes in suspension, the solid-phase RRA is significantly simpler and much faster to perform as it avoids centrifugation or filtration procedures. The solid-phase RRA was adapted profitably to process large numbers of samples at the same time. It proved particularly useful as a screening assay to detect anti-hCG monoclonal antibodies with high inhibitory activity for binding of 125I-hCG to its receptors.
已开发出一种用于测量¹²⁵I标记的人绒毛膜促性腺激素（¹²⁵I-hCG）与靶细胞膜结合的固相放射配体-受体分析（RRA）方法。¹²⁵I-hCG与固定在微量滴定板孔中的膜的结合在37℃下约3小时达到最大值，具有饱和性，显示出高亲和力（Ka = 2.4×10⁹ M⁻¹），并且可被未标记的hCG特异性抑制。与用悬浮膜进行的RRA相比，固相RRA明显更简单且执行速度快得多，因为它避免了离心或过滤程序。固相RRA适合于同时处理大量样品。事实证明，它作为一种筛选分析方法特别有用，可用于检测对¹²⁵I-hCG与其受体结合具有高抑制活性的抗hCG单克隆抗体。
