Guangdong Engineering Research Center for Insect Behavior Regulation, Key Laboratory of Bio-Pesticide Innovation and Application, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Sci Rep. 2019 Nov 1;9(1):15842. doi: 10.1038/s41598-019-52402-3.
The ATP-binding cassette (ABC) transporters exist in all living organisms and play major roles in various biological functions by transporting a wide variety of substrates across membranes. The functions of ABC transporters in drug resistance have been extensively studied in vertebrates; however, they are rarely characterized in agricultural pests. The Asian citrus psyllid, Diaphorina citri, is one of the most damaging pests of the Citrus genus because of its transmission of Huanglongbing, also known as Yellow Dragon disease. In this study, the next-generation sequencing technique was applied to research the ABC transporters of D. citri. Fifty-three ABC transporter genes were found in the RNA-Seq data, and among these ABC transporters, 4, 4, 5, 2, 1, 4, 18 and 15 ABC proteins belonged to the ABCA-ABCH subfamilies, respectively. Different expression profiles of 52 genes between imidacloprid-resistant and imidacloprid-susceptible strains were studied by qRT-PCR; 5 ABCGs and 4 ABCHs were significantly upregulated in the imidacloprid-resistant strain. In addition, five of the nine upregulated genes were widely expressed in adult tissues in spatial expression analysis. The results suggest that these genes may play key roles in this phenotype. In general, this study contributed to our current understanding of D. citri resistance to insecticides.
三磷酸腺苷结合盒（ABC）转运蛋白存在于所有生物体中，通过跨膜运输各种底物在各种生物学功能中发挥主要作用。ABC 转运蛋白在脊椎动物中的药物抗性功能已得到广泛研究，但在农业害虫中很少对其进行特征描述。亚洲柑橘木虱，Diaphorina citri，因其传播黄龙病（也称为黄龙病）而成为柑橘属中最具破坏性的害虫之一。在这项研究中，下一代测序技术被应用于研究 D. citri 的 ABC 转运蛋白。在 RNA-Seq 数据中发现了 53 个 ABC 转运蛋白基因，其中 4、4、5、2、1、4、18 和 15 个 ABC 蛋白分别属于 ABCA-ABCH 亚家族。通过 qRT-PCR 研究了 52 个基因在吡虫啉抗性和吡虫啉敏感菌株之间的不同表达谱；在吡虫啉抗性菌株中，5 个 ABCGs 和 4 个 ABCHs 显著上调。此外，在空间表达分析中，上调的 9 个基因中有 5 个在成虫组织中广泛表达。结果表明，这些基因可能在这种表型中发挥关键作用。总的来说，这项研究有助于我们目前对 D. citri 对杀虫剂抗性的理解。
