Tian Lixia, Song Tianxue, He Rongjun, Zeng Yang, Xie Wen, Wu Qingjun, Wang Shaoli, Zhou Xuguo, Zhang Youjun
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
College of Life Science, Northeast Agricultural University, Harbin, 150030, China.
BMC Genomics. 2017 Apr 26;18(1):330. doi: 10.1186/s12864-017-3706-6.
ABC transporter superfamily is one of the largest and ubiquitous groups of proteins. Because of their role in detoxification, insect ABC transporters have gained more attention in recent years. In this study, we annotated ABC transporters from a newly sequenced sweetpotato whitefly genome. Bemisia tabaci Q biotype is an emerging global invasive species that has caused extensive damages to field crops as well as ornamental plants.
A total of 55 ABC transporters containing all eight described subfamilies (A to H) were identified in the B. tabaci Q genome, including 8 ABCAs, 3 ABCBs, 6 ABCCs, 2 ABCDs, 1 ABCE, 3 ABCFs, 23 ABCGs and 9 ABCHs. In comparison to other species, subfamilies G and H in both phloem- and blood-sucking arthropods are expanded. The temporal expression profiles of these 55 ABC transporters throughout B. tabaci developmental stages and their responses to imidacloprid, a neonicotinoid insecticide, were investigated using RNA-seq analysis. Furthermore, the mRNA expression of 24 ABC transporters (44% of the total) representing all eight subfamilies was confirmed by the quantitative real-time PCR (RT-qPCR). Furthermore, mRNA expression levels estimated by RT-qPCR and RNA-seq analyses were significantly correlated (r = 0.684, p < 0.01).
It is the first genome-wide analysis of the entire repertoire of ABC transporters in B. tabaci. The identification of these ABC transporters, their temporal expression profiles during B. tabaci development, and their response to a neonicotinoid insecticide lay the foundation for functional genomic understanding of their contribution to the invasiveness of B. tabaci.
ABC转运蛋白超家族是最大且分布广泛的蛋白质家族之一。由于其在解毒过程中的作用，昆虫ABC转运蛋白近年来受到了更多关注。在本研究中，我们对新测序的烟粉虱基因组中的ABC转运蛋白进行了注释。烟粉虱Q生物型是一种新出现的全球入侵物种，已对大田作物和观赏植物造成了广泛损害。
在烟粉虱Q基因组中总共鉴定出55个ABC转运蛋白，涵盖了所有八个已知亚家族（A至H），包括8个ABCA、3个ABCB、6个ABCC、2个ABCD、1个ABCE、3个ABCF、23个ABCG和9个ABCH。与其他物种相比，韧皮部取食和吸血节肢动物中的G和H亚家族有所扩展。利用RNA测序分析研究了这55个ABC转运蛋白在烟粉虱整个发育阶段的时空表达谱及其对新烟碱类杀虫剂吡虫啉的反应。此外，通过定量实时PCR（RT-qPCR）证实了代表所有八个亚家族的24个ABC转运蛋白（占总数的44%）的mRNA表达。此外，RT-qPCR和RNA测序分析估计的mRNA表达水平显著相关（r = 0.684，p < 0.01）。
这是对烟粉虱ABC转运蛋白全基因组的首次分析。这些ABC转运蛋白的鉴定、它们在烟粉虱发育过程中的时空表达谱以及它们对新烟碱类杀虫剂的反应，为从功能基因组学角度理解它们对烟粉虱入侵性的贡献奠定了基础。
